Abstract

Secondary metabolites were isolated from cultures of four geographically distant isolates of the cosmopolitan fungus, Bipolaris sorokiniana for intense biological characterization. Analysis of extracts from their liquid and solid cultures with TLC and HPLC-MS showed high similarity of their composition despite on fungal origin. From the extracts of B. sorokiniana 14 metabolites (terpenes, quinones, xanthones etc.) were isolated using column chromatography and preparative HPLC; among them, 12 known substances were identified. Chloromonilinic acid B (CAB) showed the highest level phytotoxic activity on wheat leaves. The most of isolated compounds inhibited growth of Bacillus subtilis, of which methyl-8,3-hydroxy-3-methyl-4-chloroxanthon-1-carboxilate (MMC) demonstrated the strongest activity. Some metabolites of B. sorokiniana (CAB and MMC) were shown to have insecticidal activity against common cereal aphid. All the compounds were cytotoxic (especially, cochlioqionones and fusaroproliferin) when tested on Sf9 cell line. However, no one was acute toxic for infusoria. Evidently, the production of multiple bioactive compounds makes B. sorokiniana a strong competitor in different ecological niches and a dangerous contaminant of grain.

中文翻译：

---

不同色谱技术对双极化波氏二级代谢产物的分析和分离及其生物活性谱

摘要

次生代谢产物，从大都会真菌四个相距遥远的分离培养物中分离，蠕 sorokiniana的激烈生物学特性。用TLC和HPLC-MS对液体和固体培养物中的提取物进行分析表明，尽管它们是真菌来源，但它们的成分却具有很高的相似性。从B的提取物中提取。用柱色谱法和制备型HPLC分离索罗那氏菌14种代谢产物（萜烯，醌，氧杂蒽酮等）。其中，鉴定出12种已知物质。氯代亚麻酸B（CAB）对小麦叶片显示最高的植物毒性活性。大多数分离出的化合物抑制枯草芽孢 杆菌的生长，其中8,3-羟基-3-甲基-4-氯吨酮-1-羧酸甲酯（MMC）具有最强的活性。B的一些代谢产物。Sorokiniana（CAB和MMC）对普通谷物蚜虫具有杀虫活性。在Sf9细胞系上进行测试时，所有化合物均具有细胞毒性（尤其是耳蜗二甲酮和氟磺隆）。但是，没有人对输尿管有急性毒性。显然，生产多种生物活性化合物的使乙。sorokiniana是不同生态位的有力竞争者，也是谷物的危险污染物。