Abstract

Research on the effects of various antimicrobial agents on the strain Lactobacillus reuteri LR1 indicated its susceptibility to lincomycin, amoxicillin, and chloramphenicol, as well as resistance to other studied antibiotics. Upon milk fermentation by L. reuteri LR1 for 24 h, there was a significant increase in antioxidant and ACE-inhibitory activities, along with a decrease in L-leucine equivalents, as compared to the original milk. Further cultivation resulted in an increase in the inhibitory activity of the proteolytic, antioxidant, and angiotensin-converting enzyme (ACE), reaching its highest value after 96 h. HPLC-MS/MS analysis of the peptide profile of milk fermented by the lactobacillus showed the presence of peptides possessing ACE-inhibitory, antimicrobial, antioxidant, and immunomodulatory activities. Analysis of the substrate specificity of specificity of L. reuteri LR1 CEP proteinase toward the (f1-23) α_s1-casein fragment revealed its unique specificity, which may indicate the PI/PIII type.

中文翻译：

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罗伊氏乳杆菌LR1益生菌潜力和功能特性的体外评估

摘要

对各种抗微生物剂对路氏乳杆菌LR1的影响的研究表明，其对林可霉素，阿莫西林和氯霉素的敏感性以及对其他研究抗生素的耐药性。罗伊氏乳杆菌发酵牛奶后LR1与原始牛奶相比，在24小时内，抗氧化剂和ACE抑制活性显着增加，而L-亮氨酸当量降低。进一步培养导致蛋白水解酶，抗氧化剂和血管紧张素转化酶（ACE）的抑制活性增加，在96小时后达到最高值。乳酸菌发酵的牛奶的肽谱的HPLC-MS / MS分析表明，存在具有ACE抑制，抗微生物，抗氧化剂和免疫调节活性的肽。L特异性的底物特异性分析。罗伊氏LR1 CEP朝向（f1-23）蛋白酶α _S1酪蛋白片段揭示其独特的特异性，这可以指示PI / PIII类型。