Abstract

A method been developed for the rapid determination of bacteria on the example of E. coli cells in tap water with a slot-mode sensor in the acoustic-delay line. The method is based on the recording of changes in the depth and frequency of the resonance-absorption peaks on the frequency dependence of the output signal of the sensor before and after the infection of microbial cells with specific bacteriophages. Control experiments excluding the nonspecific interaction of microbial cells with bacteriophages were carried out. The bacterial detection limit turned out to be ~10³ cells/mL with an analysis time of 5 min. A distinctive feature of the used sensor is the presence of a removable liquid container. This allows its reuse and facilitates the process of eliminating the sample from the container.

中文翻译：

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在水生环境中检测细菌的声传感器系统

摘要

开发了一种方法，用于通过声延迟线中的缝隙模式传感器快速确定自来水中大肠杆菌细胞实例中的细菌。该方法基于在特定细菌噬菌体感染微生物细胞之前和之后，共振吸收峰的深度和频率的变化与传感器输出信号的频率相关性的记录。进行了对照实验，排除了微生物细胞与噬菌体的非特异性相互作用。细菌检测极限竟然是〜10 ³细胞/ mL，分析时间为5分钟。使用过的传感器的显着特征是存在可移动的液体容器。这允许其重复使用，并有利于从容器中清除样品的过程。