Abstract

The potential ability to cultivate a recombinant immunoglobulin (IgG1)-producer line in Iscove’s Modified Dulbecco Medium (IMDM) as the base cultivation medium with the introduction of recombinant proteins and hydrolysates of non-animal origin was studied. Recombinant insulin, human serum albumin (produced by E. coli bacteria), and transferrin (by Pichia pastoris yeasts) were used to enrich the medium. A combination of protein additives (part of the developed medium) allowed an increase in the productivity of a stable producer culture of recombinant humanized antibodies based on CHO-line cells by 44% as compared with the used complex commercial insulin–transferrin–selenium (ITS) additive. The introduction of pea and rice protein hydrolysates containing peptides with a molecular weight lower than 5 kDa to modified IMDM medium contributed to an increase in line productivity by 3.9 and 4.5 times, respectively.

中文翻译：

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基于重组蛋白添加剂和非动物源水解产物的最佳组合生产无血清培养基以生产免疫球蛋白

摘要

研究了通过引入非动物来源的重组蛋白和水解产物在作为基础培养基的Iscove改良Dulbecco培养基（IMDM）中培养重组免疫球蛋白（IgG1）-生产者系的潜在能力。重组胰岛素，人血清白蛋白（由大肠杆菌产生）和转铁蛋白（巴斯德毕赤酵母）酵母）用于丰富培养基。与使用的复杂商品化胰岛素-转铁蛋白-硒（ITS）相比，蛋白质添加剂的组合（已开发培养基的一部分）使基于CHO细胞的重组人源化抗体的稳定生产者培养的生产率提高了44％。 ）添加剂。将含有分子量低于5 kDa的肽的豌豆和大米蛋白水解物引入改良的IMDM培养基中，可分别提高生产效率3.9倍和4.5倍。