Staphylococcus aureus is one of the most common microorganisms and causes foodborne diseases. In particular, biofilm-forming S. aureus is more resistant to antimicrobial agents and sanitizing treatments than planktonic cells. Therefore, this study aimed to investigate the anti biofilm effects ofcell-free supernatant (CFS) of Saccharomyces cerevisiae isolated from cucumber jangajji compared to grapefruit seed extract (GSE). CFS and GSE inhibited and degraded S. aureus biofilms. The adhesion ability, auto-aggregation, and exopolysaccharide production of CFS-treated S. aureus, compared to those of the control, were significantly decreased. Moreover, biofilm-related gene expression was altered upon CFS treatment. Scanning electron microscopy images confirmed that CFS exerted anti-biofilm effects against S. aureus. Therefore, these results suggest that S. cerevisiae CFS has anti-biofilm potential against S. aureus strains.

中文翻译：

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酿酒酵母无细胞上清液对金黄色葡萄球菌的抗生物膜活性。

金黄色葡萄球菌是最常见的微生物之一，会引起食源性疾病。特别是，形成生物膜的金黄色葡萄球菌比浮游细胞更能抵抗抗菌剂和消毒处理。因此，本研究旨在研究从黄瓜jangajji分离的酿酒酵母无细胞上清液 (CFS)与葡萄柚籽提取物 (GSE) 相比的抗生物膜作用。CFS 和 GSE 抑制和降解金黄色葡萄球菌生物膜。CFS 处理的金黄色葡萄球菌的粘附能力、自聚集和胞外多糖产生, 与对照相比, 显着降低。此外，生物膜相关基因表达在 CFS 处理后发生了改变。扫描电子显微镜图像证实 CFS 对金黄色葡萄球菌具有抗生物膜作用。因此，这些结果表明酿酒酵母 CFS 具有针对金黄色葡萄球菌菌株的抗生物膜潜力。