Abstract

Dunaliella salina is a unicellular microalga with the potential of industrial applications. Commercial-scale cultivation of D. salina requires extensive efforts on cost reduction. This can be achieved by minimization of nutrient cost and maximization of specific growth rate and productivity. To this end, in this study, mixotrophic cultivation of D. salina on crude glycerol (by-product of calcinated-fatty acid (CaFA) production process) was investigated in shake flasks and the influences on protein, carotenoid and chlorophyll contents of the cells were studied. The highest maximum specific growth rate and biomass productivity was achieved by cultivation on 2.5 g/L crude glycerol. The protein content was more than two folds and the carotenoid content was more than 60% higher than that of autotrophic cultivation. Subsequently, continuous cultivation of D. salina on crude glycerol in a helical photobioreactor was performed at the dilution rate of 0.52/d. The biomass productivity of 2.3 × 10⁶ cell/(mL d) was achieved which was more than 75% of the values for previously reported autotrophic cultivations. Conclusively, continuous cultivation of D. salina on by-product of CaFA production process could be a potential process technology for production of D. Salina.

中文翻译：

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煅烧脂肪酸生产过程中粗制甘油对杜氏盐藻的营养综合培养

摘要

杜氏盐藻是具有工业应用潜力的单细胞微藻。商业规模的盐藻的种植需要在降低成本方面做出大量努力。这可以通过最大限度地减少营养成本以及最大化特定增长率和生产力来实现。为此，在这项研究中，盐藻的营养混合栽培在摇瓶中研究了粗甘油（煅烧脂肪酸（CaFA）生产过程的副产物）的含量，并研究了其对细胞蛋白质，类胡萝卜素和叶绿素含量的影响。通过在2.5 g / L的粗甘油上培养，可以实现最高的最大比生长速率和生物量生产率。蛋白质含量是自养植物的两倍以上，而类胡萝卜素含量则高出60％以上。随后，在螺旋光生物反应器中在粗制甘油上连续培养D. salina，稀释比为0.52 / d。生物质生产力为2.3×10 ⁶获得的细胞/（mL d）超过先前报道的自养培养的值的75％。结论是，在CaFA生产过程的副产物上连续培养D. salina可能是生产D. Salina的潜在工艺技术。