Bioactive compounds of endophytic fungus Trichoderma polyalthiae were extracted from culture broth media. The crude extracts showed strong antimicrobial activity against human pathogens. Biologically active compounds were isolated and purified by chromatographic methods. The structures of the pure compounds were elucidated by spectroscopic methods. They were identified as Violaceol I and Violaceol II. These compounds were detected as secondary metabolites produced by this genus for the first time. Violaceol I and II had a broad spectrum of antimicrobial activity against human pathogens, including Gram-positive bacteria (Staphylococcus saprophyticus, Staphylococcus aureus, Methicillin-Resistant S. aureus, Bacillus subtilis, Bacillus cereus) and Gram-negative bacteria (Salmonella typhimurium, Shigella sonnei) and Candida albicans. Violaceol I exhibited Minimal Inhibitory Concentration (MIC) values (<9.765–156.25 μg/mL) that were higher than Violaceol II (<9.765–312.5 μg/mL). Additionally, the MIC value of the phenol violaceol from this taxon was lower than the previous reports.

从培养液培养基中提取内生真菌木霉多生木霉的生物活性化合物。粗提取物显示出对人类病原体的强大抗菌活性。通过色谱方法分离和纯化生物活性化合物。通过光谱方法阐明了纯化合物的结构。它们被鉴定为紫堇醇I和紫堇醇II。这些化合物首次被检测为该属产生的次级代谢产物。Violaceol I和II具有针对人类病原体，包括革兰氏阳性菌的抗微生物活性（广谱腐生葡萄球菌，金黄色葡萄球菌，耐甲氧西林金黄色葡萄球菌，枯草芽孢杆菌，蜡状芽孢杆菌）和革兰氏阴性细菌（鼠伤寒沙门氏菌，志贺志贺菌）和白色念珠菌。Violaceol I的最低抑菌浓度（MIC）值（<9.765–156.25μg/ mL）高于Violaceol II（<9.765–312.5μg/ mL）。此外，此分类单元中酚紫杉醇的MIC值低于以前的报告。