Surface sterilization is generally used for isolating lichen-inhabiting fungi as well as endophytic fungi, and ethanol and sodium hypochlorite are commonly used as the sterilizer. However, there are few studies on whether the type of chemicals used for surface sterilization affects the isolation results of lichen-inhabiting fungi. In this study, the genus composition of the lichen-inhabiting fungi of two lichen species (Flavoparmelia caperata and Peltigera dilacerata) were investigated 1) to reveal how the isolation result changes before and after surface sterilization and 2) to examine the effect of the sterilizer (ethanol, sodium hypochlorite, or hydrogen peroxide) on the composition of the isolated fungi. We isolated 652 non-lichenized fungal isolates from the two-lichen species and identified 84 genera. It was found that 1) every sterilizer effectively removed the fungi on the lichen surface and that 2) the composition of isolated fungi varied depending on the type of surface sterilizer. It was also shown that, such as the genus Sarea, there were some lichen-inhabiting fungi which could not be isolated at all by surface sterilization with ethanol or sodium hypochlorite, which are commonly used. In addition, the genus Virgaria was detected as lichen-inhabiting fungi for the first time. Our results suggest that single surface sterilization alone may underestimate the genus composition of lichen-inhabiting fungi.

表面灭菌通常用于分离地衣类真菌和内生真菌，并且乙醇和次氯酸钠通常用作灭菌剂。但是，很少有关于用于表面灭菌的化学物质类型是否影响地衣真菌的分离结果的研究。在这项研究中，两种地衣物种（黄vo（Flavoparmelia caperata和Peltigera dilacerata））的地衣真菌的属组成）研究了1）揭示表面灭菌前后分离结果如何变化，以及2）研究了灭菌剂（乙醇，次氯酸钠或过氧化氢）对分离真菌成分的影响。我们从两个地衣物种中分离出652个非地衣真菌分离株，并鉴定出84个属。发现1）每种灭菌器均能有效清除地衣表面的真菌，2）分离的真菌的组成因表面灭菌器的类型而异。还显示出，诸如Sarea属，存在一些常存在于地衣中的真菌，这些真菌无法通过用乙醇或次氯酸钠进行表面灭菌而完全分离出来。此外，Virgaria属首次被检测为居住在地衣中的真菌。我们的结果表明，单表面灭菌可能会低估居住在地衣的真菌的属组成。