The genetic relatedness among 18 strains of Agaricus bisporus was assessed based on the fragment pattern analysis obtained by the amplification of genomic DNA by BOX, ERIC (ERICIR-I/ERIC2) and REP (REP1RI/REP2I) gene sequences. Based on the banding patterns of PCR-amplified products, eight putative groups among the 18 commercial and wild strains were recognized. REP-PCR generated multiple distinct products showing considerable variability among the strains with ERIC and REP elements successfully enabled detection of wild and commercial A. bisporus. Strains originating from the same geographical location were not always genetically related. To our knowledge, this was the first relevance study of biodiversity in commercial and native populations of A. bisporus by using the REP-PCR technique. The results confirmed the usefulness REP-PCR typing in intraspecific genetic variation assessments of the button mushroom. High level of Iranian wild strains distance with the commercial cultivars approves their importance as a promising new source of diversity in A. bisporus breeding program.

基于通过BOX，ERIC（ERICIR-1 / ERIC2）和REP（REP1RI / REP2I）基因序列扩增基因组DNA而获得的片段模式分析，评估了双孢蘑菇的18个菌株之间的遗传相关性。基于PCR扩增产物的条带模式，在18个商业和野生菌株中识别出八个推定的组。REP-PCR产生了多种不同的产物，这些产物在带有ERIC和REP元素的菌株之间表现出相当大的变异性，成功地检测了野生和商业双孢曲霉。来自相同地理位置的菌株并非总是具有遗传相关性。据我们所知，这是双孢蘑菇商业和本地种群中生物多样性的首次相关研究。通过使用REP-PCR技术。该结果证实了REP-PCR分型在蘑菇的种内遗传变异评估中的有用性。伊朗野生菌与商业品种距离的高级别批准其作为在一个多样化的有前途的新来源的重要性一。双孢菌育种程序。