The momi fir (Abies firma) has been targeted as a species for genetic resource conservation because of the recent decline in its natural populations. Additional molecular marker resources are needed to examine gene flow to understand the genetic dynamics of each population. We developed 15 genomic microsatellite (SSR) markers for A. firma and applied them to separate biparentage assignments of dispersed seeds in a natural population by assaying embryos and megagametophytes. The 15 markers were polymorphic, with the expected heterozygosity averaging greater than 0.74. Separate biparentage analysis, using the seven selected effective markers, was successful to assign paternal and maternal trees separately and thus estimate the paternal and maternal immigrant gene flow from nearby populations. The newly developed genomic SSR markers in the present study, by constructing a marker set together with previously developed markers, will provide higher discriminatory power for parentage analysis and should be useful for estimating gene flow for ongoing conservation genetics studies of A. firma.

由于近来自然种群的减少，momi fir（Abies firma）被指定为一种遗传资源保护物种。需要额外的分子标记资源来检查基因流，以了解每个群体的遗传动态。我们为A. firma开发了15个基因组微卫星（SSR）标记并通过分析胚胎和大型配子体将它们应用于自然种群中分散种子的双亲分离。这15个标记是多态性的，平均预期杂合度大于0.74。使用七个选定的有效标记进行单独的双亲分析，成功地分别分配了父本和母本树，从而估计了附近种群的父本和母本移民基因流量。通过构建与先前开发的标记一起构建的标记集，本研究中新开发的基因组SSR标记将为亲子分析提供更高的辨别力，并且应可用于估计正在进行中的A. firma保守遗传学研究的基因流量。