Background and Aims Interest in the production of wines from indigenous cultivars such as Vranac and Kratosija is steadily increasing, since they offer distinct characteristics. In contrast to popular cultivars, little is known about their chemical composition, especially in Montenegro. Many important characteristics of red wines are related to phenolic substances. Therefore, it is imperative to determine the phenolic composition of these cultivars. Methods and Results Flavan‐3‐ol monomers of seeds were analysed by reversed phase HPLC after thioacidolysis. In the perchloric extract of skins, flavan‐3‐ol monomers and dimers were determined by HPLC. Catechin represented the most abundant flavan‐3‐ol in seeds, with a high concentration of epicatechin‐3‐O‐gallate also found in Vranac. In the skins, procyanidin B1 and catechin were the most abundant phenolic substances. Using multivariate statistics, a unique profile of the ratio of flavan‐3‐ols from each cultivar was found. Conclusions Cultivar and geographical origin considerably influenced the flavan‐3‐ol composition of the three cultivars. The flavan‐3‐ol concentration of berries exhibited characteristic varietal differences, with the order of concentration being Cabernet Sauvignon > Kratosija > Vranac. The indigenous Montenegrin cultivars had a significantly lower proportion of seed and skin mass than that of Cabernet Sauvignon, which resulted in a lower flavan‐3‐ol concentration in the berries. Significance of the Study The flavan‐3‐ol concentration of grapes from Vranac, Kratosija and Cabernet Sauvignon cultivars grown in Montenegro was determined for the first time. These results further broaden knowledge relating to characterisation of wine, optimisation of its production and authenticity control.

中文翻译：

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flavan-3-ol 分析用于区分葡萄与黑山生长的 Vranac、Kratošija 和 Cabernet Sauvignon ( Vitis vinifera L.) 的适用性

背景和目标 人们对用 Vranac 和 Kratosija 等本土品种生产葡萄酒的兴趣正在稳步增加，因为它们具有鲜明的特征。与流行的品种相比，人们对其化学成分知之甚少，尤其是在黑山。红葡萄酒的许多重要特性都与酚类物质有关。因此，确定这些品种的酚类成分势在必行。方法和结果 硫代酸解后，通过反相 HPLC 分析种子的 Flavan-3-ol 单体。在皮肤的高氯酸提取物中，黄烷-3-醇单体和二聚体通过 HPLC 测定。儿茶素代表了种子中最丰富的 flavan-3-ol，在 Vranac 中也发现了高浓度的表儿茶素-3-O-没食子酸酯。在皮肤中，原花青素 B1 和儿茶素是最丰富的酚类物质。使用多变量统计数据，发现了来自每个栽培品种的 flavan-3-ols 比率的独特特征。结论 品种和地理起源对三个品种的 flavan-3-ol 组成有很大影响。浆果的 flavan-3-ol 浓度表现出特征性的品种差异，浓度顺序为 Cabernet Sauvignon > Kratosija > Vranac。与赤霞珠相比，黑山本土品种的种子和表皮质量比例显着降低，导致浆果中黄烷-3-醇的浓度较低。研究的意义首次测定了黑山种植的 Vranac、Kratosija 和 Cabernet Sauvignon 葡萄的 flavan-3-ol 浓度。