The aim of this work is to study the photochemical processes of hydrolysis of biomacromolecules, using the example of DNA in DNA and DNA-Polyethylene glycol (model of nucleoprotein) solutions. The processes induced by the line spectrum of an argon glow discharge lamp (AGDL) (700–1800 nm, 1.7–0.7 eV) have been studied by spectrophotometry, light scattering, turbidimetry, and spectrofluorimetry. It is shown that acridine orange (AO) intercalated in DNA is a good indicator of pH, i.e. of the presence of H 3 O + ions in DNA solutions. H 3 O + ions quench AO fluorescence and, in addition, eject it from the DNA in the solution. Using the methods of laser spectroscopy (light scattering, fluorescence, and resonance energy transfer) and spectrophotometry, we have observed hydrolysis of water solutions and of glycosidic and phosphodiester bonds in DNA and DNA-polyethylene glycol water solutions induced by near-infrared (700–1800 nm, (AGDL)) irradiatio...

中文翻译：

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激光光谱法研究氩辉光放电对DNA分子构象的影响

这项工作的目的是，以DNA和DNA-聚乙二醇（核蛋白模型）溶液中的DNA为例，研究生物大分子水解的光化学过程。通过分光光度法，光散射，比浊法和荧光分光光度法研究了由氩辉光放电灯（AGDL）（700-1800 nm，1.7-0.7 eV）的线谱引起的过程。结果表明，插入到DNA中的a啶橙（AO）可以很好地指示pH值，即DNA溶液中H 3 O +离子的存在。H 3 O +离子猝灭AO荧光，此外，还可以将其从溶液中的DNA中弹出。使用激光光谱法（光散射，荧光和共振能量转移）和分光光度法，