Organ-on-chip models, known as microphysiological systems, are created to mimic the anatomy and physiology of a human organ at the micro-level. Besides being pivotal in the reverse engineering of human organs and pathogenesis studies, they serve as an alternative to animal testing and the development of pharmaceutics. Monitoring the extracellular stromal environment is the basis for gaining in-depth knowledge of the pathophysiology of cell culture. Hence, it is extensively employed as an essential tool in the fields of organ-on-chip and in vitro toxicology. In this study, we explore the vitality of a microfluidic system for the automated, online detection of drug-induced physical changes in cellular viability by continual monitoring of a microfluidic 2D monolayer cell culture. Trans-epithelial electrical resistance (TEER) values and pH changes of the immortal HepG2 cell line were measured continuously using microfluidic-based electrical and photoelectric sensors. A chip-e...

中文翻译：

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基于实时生理传感器的片上肝脏监测药物毒性的装置

创建了称为微生理系统的芯片上器官模型，以在微观级别模拟人体器官的解剖结构和生理。除了在人体器官的逆向工程和发病机理研究中发挥关键作用外，它们还可以替代动物实验和药物开发。监测细胞外基质环境是获得细胞培养病理生理学深入知识的基础。因此，它被广泛用作芯片上器官和体外毒理学领域的基本工具。在这项研究中，我们通过持续监控微流体2D单层细胞培养物，探索了微流体系统的生命力，该系统可自动在线检测药物诱导的细胞生存力的物理变化。使用基于微流体的电和光电传感器连续测量永生HepG2细胞系的跨上皮电阻（TEER）值和pH变化。芯片芯片