The present study reports the antibacterial potential of phyto-nano-hybrid particles Ag-CuO (silver-copper oxide) against drug-resistant pathogens isolated from a Russian hospital in Krasnoyarsk, Siberia. The synthesis of nano-hybrid was achieved by phytogenic source by using leaves of Murraya koenigii. The nano-hybrid particles were well characterized using hyphenated techniques and results of the antibacterial assay was tabulated. The UV-visible spectra displayed absorption at 420 nm with the shoulder peak at 355 nm indicating the hybridization. The FTIR analysis revealed the presence of phenol, amine, methyl, carbohydrate and aromatic as major functional groups. The XRD analysis revealed the presence of Bragg’s intensities at 2 theta angle depicting the crystalline nature of Ag-CuO nano-hybrid. The TEM analysis displayed the polydispered properties of Ag-CuO nano-hybrid with the size in the range of 60–80 nm exhibiting different shapes ranging from spherical, rod and oval. The antibacterial activity of Ag-CuO nano-hybrid was tested against multidrug-resistant pathogens that resulted in highest activity against P. aeruginosa strain with an inhibition zone of 14 mm in diameter. The MIC concentrations ranged from 0.3125 to 2.5 μg/ml and broth dilution assay displayed dose-dependent properties of Ag-CuO nano-hybrid particles. The obtained results are interesting to report the preliminary insight to develop biocompatible hybrid particles to combat drug-resistant pathogens. The developed nano-hybrid particles displayed activity against all the test pathogens investigated against both Gram-positive and Gram-negative bacteria. Thus, the study forms preliminary investigation to report nano-hybrid particles as broad spectrum antibacterial agents.

中文翻译：

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Ag-CuO颗粒的植物-纳米杂合体，具有抗药原性病原体的抗菌活性。

本研究报告了植物纳米杂化颗粒Ag-CuO（银铜氧化物）对从西伯利亚克拉斯诺亚尔斯克一家俄罗斯医院分离出的耐药病原体的抗菌潜力。纳米杂化的合成是通过使用植物来源的Murraya koenigii的叶子来实现的。纳米杂化的粒子已使用连接技术很好地表征，并列出了抗菌测定的结果。紫外可见光谱显示在420 nm处有吸收，肩峰在355 nm处表明杂交。FTIR分析表明存在酚，胺，甲基，碳水化合物和芳族化合物作为主要官能团。XRD分析表明在2θ角处存在布拉格强度，其描述了Ag-CuO纳米杂化体的晶体性质。TEM分析显示，Ag-CuO纳米杂化体的多分散性质为60-80 nm，呈现出球形，棒状和椭圆形等不同形状。测试了Ag-CuO纳米杂化物对多种药物耐药病原体的抗菌活性，这些病原体对铜绿假单胞菌菌株具有最高的抑制作用，直径为14 mm。MIC浓度范围为0.3125至2.5μg/ ml，肉汤稀释试验显示出Ag-CuO纳米杂化颗粒的剂量依赖性。所获得的结果很有趣，可用于报告初步的见识，以开发生物相容性杂化颗粒来对抗耐药病原体。发达的纳米杂化颗粒显示出针对所有针对革兰氏阳性和革兰氏阴性细菌的测试病原体的活性。从而，