Accessible chromatin plays a central role in gene expression and chromatin architecture. Current accessible chromatin approaches depend on limited digestion/cutting and pasting adaptors at the accessible DNA, thus requiring additional materials and time for optimization. Universal NicE-seq (UniNicE-seq) is an improved accessible chromatin profiling method that negates the optimization step and is suited to a variety of mammalian cells and tissues. Addition of 5-methyldeoxycytidine triphosphate during accessible chromatin labeling and an on-bead library making step substantially improved the signal to noise ratio while protecting the accessible regions from repeated nicking in cell lines, mouse T cells, mouse kidney, and human frozen tissue sections. We also demonstrate one tube UniNicE-seq for the FFPE tissue section for direct NGS library preparation without sonication and DNA purification steps. These refinements allowed reliable mapping of accessible chromatin for high-resolution genomic feature studies.

中文翻译：

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通用 NicE-seq，可对甲醛固定和 FFPE 组织进行高分辨率、可访问的染色质分析。

可接近的染色质在基因表达和染色质结构中起着核心作用。目前可访问的染色质方法依赖于可访问 DNA 上有限的消化/切割和粘贴接头，因此需要额外的材料和时间进行优化。Universal NicE-seq (UniNicE-seq) 是一种改进的可访问染色质分析方法，无需优化步骤，适用于多种哺乳动物细胞和组织。在可及染色质标记和珠上文库制作步骤中添加 5-甲基脱氧胞苷三磷酸可显着提高信噪比，同时保护可及区域免受细胞系、小鼠 T 细胞、小鼠肾脏和人类冷冻组织切片中的重复切口。我们还展示了用于 FFPE 组织切片的一管 UniNicE-seq，用于直接 NGS 文库制备，无需超声处理和 DNA 纯化步骤。这些改进可以为高分辨率基因组特征研究提供可靠的染色质图谱。