The ability to deliver foreign cargos into single living cells is of great interest in cell biology and therapeutic research. Here, we have reported a single or multiple position based nano-localized single-cell nano-electroporation platform. The device consists of an array of triangular shape ITO nano-electrodes with a 70 nm gap between two nano-electrodes, each having a 40 nm tip diameter. The voltage is applied between nano-electrodes to generate an intense electric field, which electroporates multiple nano-localized regions of the targeted single-cell membrane, and biomolecules are gently delivered into cells by pressurizing pump flow, without affecting cell viability. The platform successfully delivers dyes, QDs, and plasmids into different cell types with the variation of field strength, pulse duration, and the number of pulses. This new approach allows us to analyze delivery of different biomolecules into single living cells with high transfection efficiency (>96%, for CL1-0 cells) and high cell viability (∼98%), which are potentially beneficial for cellular therapy and diagnostic purposes.

中文翻译：

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纳米定位单细胞纳米电穿孔

将异物运送到单个活细胞中的能力在细胞生物学和治疗研究中引起了极大的兴趣。在这里，我们报告了一个或多个基于位置的纳米定位单细胞纳米电穿孔平台。该设备由三角形的ITO纳米电极阵列组成，两个纳米电极之间的间隙为70 nm，每个纳米电极的尖端直径为40 nm。在纳米电极之间施加电压以产生强电场，该电场使目标单细胞膜的多个纳米定位区域电穿孔，并且通过加压泵流将生物分子缓慢递送到细胞中，而不会影响细胞生存力。该平台可根据场强，脉冲持续时间和脉冲数的变化，成功地将染料，QD和质粒传递到不同的细胞类型中。