Compelling evidence from basic molecular biology has demonstrated the crucial role of microglia in the pathogenesis of Alzheimer’s disease (AD). Microglia were believed to play a dual role in both promoting and inhibiting Alzheimer’s disease progression. It is of great significance to regulate the function of microglia and make them develop in a favorable way. In the present study, we investigated the function of repressor element 1-silencing transcription factor (REST) in Aβ_1-42-induced BV-2 cell dysfunction. We concluded that Aβ_1-42 could promote type I activation of BV-2 cells and induce cell proliferation, migration, and proinflammation cytokine TNF-α, IL-1β, and IL-6 expression. Meanwhile, REST was upregulated, and nuclear translocalization took place due to Aβ_1-42 stimulation. When REST was knocked down by a specific short hairpin RNA (sh-RNA), BV-2 cell proliferation, migration, and proinflammation cytokine expression and secretion induced by Aβ_1-42 were increased, demonstrating that REST may act as a repressor of microglia-like BV-2 cell activation.

中文翻译：

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Aβ 诱导的阻遏元件 1-沉默转录因子 (REST) 基因传递抑制小胶质细胞样 BV-2 细胞的激活

来自基础分子生物学的令人信服的证据证明了小胶质细胞在阿尔茨海默病 (AD) 的发病机制中的关键作用。小胶质细胞被认为在促进和抑制阿尔茨海默病进展方面发挥双重作用。调节小胶质细胞的功能，使其有利于发育，具有重要意义。在本研究中，我们研究了阻遏元件 1 沉默转录因子 (REST) 在 A β _1-42诱导的 BV-2 细胞功能障碍中的功能。我们得出结论，A β _1-42可以促进 BV-2 细胞的 I 型活化并诱导细胞增殖、迁移和促炎细胞因子 TNF - α、IL-1 β, 和 IL-6 表达。同时，REST 被上调，并且由于 A β _1-42刺激发生核转位。当 REST 被特定的短发夹 RNA (sh-RNA) 击倒时，A β _1-42诱导的 BV-2 细胞增殖、迁移和促炎细胞因子表达和分泌增加，表明 REST 可能作为小胶质细胞样 BV-2 细胞活化。