Ultraviolet photoacoustic microscopy (UV-PAM) has recently been demonstrated as a potential imaging tool for surgical margin analysis (SMA). UV-PAM does not require staining or micrometer-thick slicing, which is inevitable in conventional histological imaging. To promote UV-PAM as a practical intraoperative diagnostic tool, the imaging speed should be improved while preserving the high-resolution imaging capability and simplistic system design. In this Letter, we developed a galvanometer mirror-based UV-PAM (GM-UV-PAM) system for high-speed histology-like imaging. By using a UV laser with a high repetition rate (55 kHz) and a one-dimensional galvanometer mirror, our GM-UV-PAM system can generate subcellular images in less than 15 min for a typical brain biopsy (${{5}}\;{\rm{mm}} \times {{5}}\;{\rm{mm}}$), with a lateral resolution of ${\sim}{1.0}\;\unicode{x00B5}{\rm m}$. The images of mouse brain slices obtained by our GM-UV-PAM system show that it can provide histological information for SMA.

中文翻译：

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高速无标记紫外光声显微镜，用于未经处理的生物组织的组织学样成像

紫外线光声显微镜（UV-PAM）最近已被证明是用于手术切缘分析（SMA）的潜在成像工具。UV-PAM不需要染色或千分尺厚的切片，这在常规组织学成像中是不可避免的。为了将UV-PAM推广为一种实用的术中诊断工具，应在保持高分辨率成像能力和简化系统设计的同时提高成像速度。在这封信中，我们开发了基于振镜反射镜的UV-PAM（GM-UV-PAM）系统，用于高速组织学样成像。通过使用具有高重复频率（55 kHz）的UV激光器和一维振镜，我们的GM-UV-PAM系统可以在不到15分钟的时间内完成典型的脑活检的亚细胞图像（$ {{5}} \; {\ rm {mm}} \ times {{5}} \; {\ rm {mm}} $），横向分辨率为$ {\ sim} {1.0} \ ;; \ unicode {x00B5} {\ rm m} $。通过我们的GM-UV-PAM系统获得的小鼠脑切片图像显示，它可以为SMA提供组织学信息。