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Anti-Parkinson’s Activity of Tribulus terrestris via Modulation of AChE, α-Synuclein, TNF-α, and IL-1β
ACS Omega ( IF 3.7 ) Pub Date : 2020-09-22 , DOI: 10.1021/acsomega.0c03375 Uzma Saleem 1 , Zunera Chauhdary 1 , Zohaib Raza 1 , Shahid Shah 2 , Mahmood-ur Rahman 3 , Parwasha Zaib 3 , Bashir Ahmad 4
ACS Omega ( IF 3.7 ) Pub Date : 2020-09-22 , DOI: 10.1021/acsomega.0c03375 Uzma Saleem 1 , Zunera Chauhdary 1 , Zohaib Raza 1 , Shahid Shah 2 , Mahmood-ur Rahman 3 , Parwasha Zaib 3 , Bashir Ahmad 4
Affiliation
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Tribulus terrestris (T.T.) is a rich source of flavonoids and saponins, which have been reported to have neuroprotective and antioxidant potential. The current study was planned to investigate the anti-Parkinson’s activity of T. terrestris methanol extract (TTME). It was hypothesized that TTME possessed antioxidant potential and can ameliorate Parkinson’s disease (PD) via modulation of α-synuclein, acetylcholinesterase (AChE), TNF-α, and IL-1β. To test this hypothesis, in silico and in vivo studies were performed. The PD model in rats was prepared by giving haloperidol, 1 mg/kg, i.p. Rats were divided into six groups: control, disease control, standard, and treatment groups receiving TTME orally at 100, 300, and 1000 mg/kg dose levels for 21 days. Behavioral observations and biochemical analyses were done. The TTME modulatory effect on mRNA expression of α-synuclein, AChE, TNF-α, and interleukins in the brain homogenate was estimated by RT-PCR. Compounds detected in HPLC analysis disrupted the catalytic triad of AChE in in silico studies. Behavioral observations showed significant (p < 0.05) improvement in a reversal of catatonia, muscular strength, locomotor functions, stride length, and exploration in a dose-dependent manner (1000 >300 >100 mg/kg) of PD rats. Endogenous antioxidant enzyme levels CAT, SOD, GSH, and GPx were significantly restored at a high dose (p < 0.05) with a notable (p < 0.05) decrease in the MDA level in TTME-treated groups. TTME at a high dose significantly (p < 0.05) decreased the level of acetylcholinesterase. RT-PCR results are showing down-regulation in the mRNA expression levels of IL-1β, α -synuclein, TNF-α, and AChE in TTME-treated groups compared to the disease control group, indicating neuroprotection. It is concluded that TTME has potential to ameliorate the symptoms of Parkinson’s disease.
中文翻译：
的抗帕金森病活性的蒺藜通过乙酰胆碱酯酶的调制，α突触核蛋白，TNF-α和IL-1β
bul藜（TT）是类黄酮和皂苷的丰富来源，据报道它们具有神经保护和抗氧化作用。目前的研究计划调查T. terrestris的抗帕金森氏菌活性甲醇萃取液（TTME）。假设TTME具有抗氧化能力，并且可以通过调节α-突触核蛋白，乙酰胆碱酯酶（AChE），TNF-α和IL-1β来改善帕金森氏病（PD）。为了验证该假设，进行了计算机和体内研究。通过给予氟哌啶醇1 mg / kg ip制备大鼠PD模型，将其分为六组：对照组，疾病对照组，标准组和治疗组，口服TTME剂量分别为100、300和1000 mg / kg， 21天 进行了行为观察和生化分析。通过RT-PCR估计TTME对脑匀浆中α-突触核蛋白，AChE，TNF-α和白介素mRNA表达的调节作用。HPLC分析中检测到的化合物在计算机研究中破坏了AChE的催化三联体。行为观察表明，p <0.05）改善了大鼠的紧张程度，肌肉力量，运动功能，步幅和以剂量依赖方式（1000> 300> 100 mg / kg）进行的探索。内源性抗氧化酶水平CAT，SOD，GSH和GPx在高剂量下（p <0.05）显着恢复，而TTME治疗组的MDA水平显着下降（p <0.05）。高剂量的TTME显着降低（p <0.05）乙酰胆碱酯酶的水平。RT-PCR结果显示，与疾病对照组相比，TTME治疗组中IL-1β，α-突触核蛋白，TNF-α和AChE的mRNA表达水平下调，表明其具有神经保护作用。结论是，TTME具有改善帕金森氏病症状的潜力。
更新日期：2020-10-06
中文翻译：
的抗帕金森病活性的蒺藜通过乙酰胆碱酯酶的调制，α突触核蛋白，TNF-α和IL-1β
bul藜（TT）是类黄酮和皂苷的丰富来源，据报道它们具有神经保护和抗氧化作用。目前的研究计划调查T. terrestris的抗帕金森氏菌活性甲醇萃取液（TTME）。假设TTME具有抗氧化能力，并且可以通过调节α-突触核蛋白，乙酰胆碱酯酶（AChE），TNF-α和IL-1β来改善帕金森氏病（PD）。为了验证该假设，进行了计算机和体内研究。通过给予氟哌啶醇1 mg / kg ip制备大鼠PD模型，将其分为六组：对照组，疾病对照组，标准组和治疗组，口服TTME剂量分别为100、300和1000 mg / kg， 21天 进行了行为观察和生化分析。通过RT-PCR估计TTME对脑匀浆中α-突触核蛋白，AChE，TNF-α和白介素mRNA表达的调节作用。HPLC分析中检测到的化合物在计算机研究中破坏了AChE的催化三联体。行为观察表明，p <0.05）改善了大鼠的紧张程度，肌肉力量，运动功能，步幅和以剂量依赖方式（1000> 300> 100 mg / kg）进行的探索。内源性抗氧化酶水平CAT，SOD，GSH和GPx在高剂量下（p <0.05）显着恢复，而TTME治疗组的MDA水平显着下降（p <0.05）。高剂量的TTME显着降低（p <0.05）乙酰胆碱酯酶的水平。RT-PCR结果显示，与疾病对照组相比，TTME治疗组中IL-1β，α-突触核蛋白，TNF-α和AChE的mRNA表达水平下调，表明其具有神经保护作用。结论是，TTME具有改善帕金森氏病症状的潜力。
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