G-quadruplexes are non-canonical four stranded secondary structures possessing great biological importance. Controlling G-quadruplex conformation for further regulating biological processes is both exciting and challenging. In this study, we described a method for regulating G-quadruplex conformation by click chemistry for the first time. 8-ethynyl-2′-deoxyguanosine was synthesized and incorporated into a 12-nt telomere DNA sequence. Such a sequence, at first, formed mixed parallel/anti-parallel G-quadruplexes, while it changed to anti-parallel after reaction with azidobenzene. Meanwhile, the click reaction can give the sequence intense fluorescence.

中文翻译：

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点击反应控制的G-四链体构象

G-四链体是非规范的四链二级结构，具有重要的生物学意义。控制 G-四链体构象以进一步调节生物过程既令人兴奋又具有挑战性。在这项研究中，我们首次描述了一种通过点击化学调节 G-四链体构象的方法。合成了 8-乙炔基-2'-脱氧鸟苷并将其整合到 12-nt 端粒 DNA 序列中。这样的序列首先形成混合的平行/反平行G-四链体，而在与叠氮苯反应后变为反平行。同时，点击反应可以使序列产生强烈的荧光。