Liver ischaemia reperfusion injury (IRI) is a dreaded pathophysiological complication which may lead to an impaired liver function. The level of oxygen hypoperfusion affects the level of cellular damage during the reperfusion phase. Consequently, intraoperative localisation and quantification of oxygen impairment would help in the early detection of liver ischaemia. To date, there is no real-time, non-invasive, and intraoperative tool which can compute an organ oxygenation map, quantify and discriminate different types of vascular occlusions intraoperatively. Hyperspectral imaging (HSI) is a non-invasive optical methodology which can quantify tissue oxygenation and which has recently been applied to the medical field. A hyperspectral camera detects the relative reflectance of a tissue in the range of 500 to 1000 nm, allowing the quantification of organic compounds such as oxygenated and deoxygenated haemoglobin at different depths. Here, we show the first comparative study of liver oxygenation by means of HSI quantification in a model of total vascular inflow occlusion (VIO) vs. hepatic artery occlusion (HAO), correlating optical properties with capillary lactate and histopathological evaluation. We found that liver HSI could discriminate between VIO and HAO. These results were confirmed via cross-validation of HSI which detected and quantified intestinal congestion in VIO. A significant correlation between the near-infrared spectra and capillary lactate was found (r = − 0.8645, p = 0.0003 VIO, r = − 0.7113, p = 0.0120 HAO). Finally, a statistically significant negative correlation was found between the histology score and the near-infrared parameter index (NIR) (r = − 0.88, p = 0.004). We infer that HSI, by predicting capillary lactates and the histopathological score, would be a suitable non-invasive tool for intraoperative liver perfusion assessment.

肝缺血再灌注损伤 (IRI) 是一种可怕的病理生理并发症，可能导致肝功能受损。氧低灌注水平影响再灌注阶段的细胞损伤水平。因此，术中氧损伤的定位和量化将有助于早期发现肝脏缺血。迄今为止，还没有实时、非侵入性和术中工具可以计算器官氧合图，量化和区分术中不同类型的血管闭塞。高光谱成像 (HSI) 是一种可以量化组织氧合的非侵入性光学方法，最近已应用于医学领域。高光谱相机检测组织在 500 到 1000 nm 范围内的相对反射率，允许量化有机化合物，例如不同深度的含氧和脱氧血红蛋白。在这里，我们通过 HSI 量化在总血管流入闭塞 (VIO) 与肝动脉闭塞 (HAO) 模型中展示了肝脏氧合的第一次比较研究，将光学特性与毛细血管乳酸和组织病理学评估相关联。我们发现肝脏 HSI 可以区分 VIO 和 HAO。这些结果通过 HSI 的交叉验证得到证实，HSI 检测和量化 VIO 中的肠充血。发现近红外光谱与毛细管乳酸之间存在显着相关性（r = - 0.8645，p = 0.0003 VIO，r = - 0.7113，p = 0.0120 HAO）。最后，发现组织学评分和近红外参数指数 (NIR) 之间存在统计学上显着的负相关（r = - 0.88，p = 0.004）。我们通过预测毛细血管乳酸和组织病理学评分推断 HSI 将是一种合适的非侵入性工具，用于术中肝脏灌注评估。