当前位置：
X-MOL 学术
›
ACS Biomater. Sci. Eng.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Theranostic Applications of Nanostructured Silicate-Substituted Hydroxyapatite Codoped with Eu3+ and Bi3+ Ions—A Novel Strategy for Bone Regeneration
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-09-22 , DOI: 10.1021/acsbiomaterials.0c00824 Sara Targonska 1 , Mateusz Sikora 2 , Krzysztof Marycz 2, 3, 4 , Agnieszka Smieszek 2 , Rafal J. Wiglusz 1, 5
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-09-22 , DOI: 10.1021/acsbiomaterials.0c00824 Sara Targonska 1 , Mateusz Sikora 2 , Krzysztof Marycz 2, 3, 4 , Agnieszka Smieszek 2 , Rafal J. Wiglusz 1, 5
Affiliation
|
In this paper, nanocrystalline silicate-substituted hydroxyapatites (nSi-HAps) codoped with Eu3+ were functionalized with Bi3+ ions. Biomaterials were synthesized using a microwave-assisted hydrothermal method and heat-treated at 700 °C. The concentration of Eu3+ ions was established at 1 mol %, and the concentration of Bi3+ was in the range of 0.5–2 mol %. The physicochemical properties of the obtained biomaterials were determined using previously established methods, including X-ray powder diffraction, scanning electron microscopy techniques, and IR spectroscopy. Particle sizes obtained in this study were in the range of 22–65 nm, which was established by the Rietveld method. The luminescence properties of the Eu3+ ion-doped silicate-substituted apatite were recorded depending on the bismuth(III) concentration. The cytocompatibility of obtained biomaterials was tested using the model of mouse pre-osteoblasts cell line, that is, MC3T3-E1. We showed that the obtained biomaterials exerted anti-apoptotic effect, reducing the number of early and late apoptotic cells and decreasing caspase activity and reactive oxygen species accumulation. The transcripts levels of genes associated with apoptosis confirmed the anti-apoptotic effect of the biomaterials. Increased metabolic activity of MC3T3-E1 in cultures with biomaterials functionalized with Bi3+ ions has been observed. Moreover, the determined profile of osteogenic markers indicates that the obtained matrices, that is, Eu3+:nSi-HAp functionalized with Bi3+ ions, exert pro-osteogenic properties. The biological features of Eu3+:nSi-HAp modified with Bi3+ ions are highly desired in terms of functional tissue restoration and further efficient osteointegration.
中文翻译：
Eu 3+和Bi 3+离子共掺杂的纳米结构硅酸盐替代羟基磷灰石的分子筛学应用-一种骨再生的新策略
在本文中，用Bi 3+离子对掺有Eu 3+的纳米晶硅酸盐取代的羟基磷灰石（nSi-HAps）进行了功能化。使用微波辅助水热法合成生物材料，并在700°C下进行热处理。Eu 3+离子的浓度为1 mol％，Bi 3+的浓度为0.5-2 mol％。使用先前建立的方法，包括X射线粉末衍射，扫描电子显微镜技术和IR光谱法，确定了获得的生物材料的理化性质。在这项研究中获得的粒径在22–65 nm范围内，这是通过Rietveld方法确定的。Eu 3+的发光特性根据铋（III）的浓度记录了离子掺杂的硅酸盐取代的磷灰石。使用小鼠成骨细胞前体细胞系MC3T3-E1的模型测试获得的生物材料的细胞相容性。我们表明，获得的生物材料发挥抗凋亡作用，减少早期和晚期凋亡细胞的数量，并降低胱天蛋白酶活性和活性氧的积累。与细胞凋亡相关的基因的转录水平证实了生物材料的抗凋亡作用。已观察到在具有被Bi 3+离子功能化的生物材料的培养物中，MC3T3-E1的代谢活性增加。此外，确定的成骨标记物特征表明所获得的基质，即Eu 3+：nSi-HAp被Bi 3+离子官能化，具有促成骨作用。就功能组织修复和进一步有效的骨整合而言，非常需要用Bi 3+离子修饰的Eu 3+：nSi-HAp的生物学特征。
更新日期：2020-11-09
中文翻译：
Eu 3+和Bi 3+离子共掺杂的纳米结构硅酸盐替代羟基磷灰石的分子筛学应用-一种骨再生的新策略
在本文中，用Bi 3+离子对掺有Eu 3+的纳米晶硅酸盐取代的羟基磷灰石（nSi-HAps）进行了功能化。使用微波辅助水热法合成生物材料，并在700°C下进行热处理。Eu 3+离子的浓度为1 mol％，Bi 3+的浓度为0.5-2 mol％。使用先前建立的方法，包括X射线粉末衍射，扫描电子显微镜技术和IR光谱法，确定了获得的生物材料的理化性质。在这项研究中获得的粒径在22–65 nm范围内，这是通过Rietveld方法确定的。Eu 3+的发光特性根据铋（III）的浓度记录了离子掺杂的硅酸盐取代的磷灰石。使用小鼠成骨细胞前体细胞系MC3T3-E1的模型测试获得的生物材料的细胞相容性。我们表明，获得的生物材料发挥抗凋亡作用，减少早期和晚期凋亡细胞的数量，并降低胱天蛋白酶活性和活性氧的积累。与细胞凋亡相关的基因的转录水平证实了生物材料的抗凋亡作用。已观察到在具有被Bi 3+离子功能化的生物材料的培养物中，MC3T3-E1的代谢活性增加。此外，确定的成骨标记物特征表明所获得的基质，即Eu 3+：nSi-HAp被Bi 3+离子官能化，具有促成骨作用。就功能组织修复和进一步有效的骨整合而言，非常需要用Bi 3+离子修饰的Eu 3+：nSi-HAp的生物学特征。
京公网安备 11010802027423号