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Virulence of Purpureocillium lilacinum strain ZJPL08 and efficacy of a wettable powder formulation against the Asian citrus psyllid (Diaphorina citri)
Biotechnology & Biotechnological Equipment ( IF 1.5 ) Pub Date : 2020-09-22
Danchao Du, Lianming Lu, Xiurong Hu, Zhanxu Pu, Zhendong Huang, Guoqing Chen, Shunmin Liu, Jia Lyu

The Asian citrus psyllid, Diaphorina citri Kuwayama, is one of the most destructive pests of citrus and the primary vector of Candidatus Liberibacter spp., which cause citrus Huanglongbing (HLB). Microbial insecticides based on entomopathogenic fungi are safer alternatives to chemical pesticides. They can mitigate the spread of HLB by suppressing D. citri populations in citrus groves. Purpureocillium lilacinum strain ZJPL08, previously isolated from D. citri cadavers, is highly pathogenic to D. citri. We determined the virulence of strain ZJPL08 conidial suspensions and the efficacy of a wettable powder formulation. The LC50 of strain ZJPL08 against D. citri at 7 d post-inoculation was 2.11 × 104 conidia/mL, and the LT50 of a 1.0 × 108 conidia/mL suspension was 3.14 d in the laboratory. Under the same conditions, the LC50 of the wettable powder was 1.39 × 104 conidia/mL, and the LT50 was 2.64 d. The corrected mortality caused by the 1500-times diluted solutions of strain ZJPL08 wettable powder in D. citri reached 86.67% at 7 d post-treatment and 100% at 14 d post-treatment under field conditions. Our study revealed that strain ZJPL08 infected D. citri by hyphae penetrating directly through the cuticle and into the body cavity and tissues. These findings show that P. lilacinum strain ZJPL08 has potential for the effective control of D. citri.



中文翻译:

淡紫色紫癜菌ZJPL08的毒力和可湿性粉剂对亚洲柑橘木虱(Diaphorina citri)的功效

的亚洲柑橘木虱,柑橘木虱桑山,是柑橘的最具破坏性的害虫和的初级矢量之一暂定Liberibacter属,其原因柑橘黄龙病(HLB)。基于昆虫病原性真菌的微生物杀虫剂是化学农药的更安全替代品。它们可以通过抑制柑橘林中的柑橘D. citri种群来减轻HLB的传播。紫癜紫丁香菌株ZJPL08,先前从D分离。柠檬尸体,对D具有很高的致病性。红蜘蛛。我们确定了菌株ZJPL08分生孢子悬浮液的毒性和可湿性粉剂的功效。LCD的ZJPL08菌株50。接种后7 d的柠檬酸为2.11×10 4分生孢子/ mL,在实验室中1.0×10 8分生孢子/ mL悬浮液的LT 50为3.14 d。在相同条件下,可湿性粉剂的LC 50为1.39×10 4分生孢子/ mL,LT 50为2.64 d。D菌株ZJPL08可湿性粉剂1500倍稀释溶液引起的校正死亡率。在田间条件下,柠檬酸在处理后第7天达到86.67%,在处理后第14天达到100%。我们的研究表明,应变ZJPL08感染d柠檬通过菌丝直接穿透角质层并进入体腔和组织。这些发现表明,丁香假单胞菌菌株ZJPL08具有有效控制D的潜力。红蜘蛛

更新日期:2020-09-22
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