Pulmonary arterial hypertension (PAH) is a life‐threatening disease characterized by a constant high pulmonary artery pressure and the remodeling of the vessel. Chloroquine (CLQ) has been observed to inhibit calcium influx. The aim of this study is to investigate the effect of CLQ on transient receptor cationic proteins (TRPC1 and TRPC6) and extracellular calcium‐sensitive receptor (CaSR) in a hypoxic PAH model. In this study, 8‐ to 12‐week‐old 32 male Wistar albino rats, weighing 200 to 300 g, were used. The rats were studied in four groups, including normoxy control, n = 8; normoxy CLQ (50 mg/kg/28 d), n = 8; hypoxia (HX; 10% oxygen/28 d) control, n = 8; and HX (10% oxygen/28 d) + CLQ (50 mg/kg), N = 8. Pulmonary arterial medial wall thickness, pulmonary arteriole wall, TRPC1, TRPC6, and CaSR expressions were evaluated by immunohistochemistry, polymerase chain reaction, and enzyme‐linked immunosorbent assay methods. At the end of the experiment, a statistically significant increase in the medial wall thickness was observed in the hypoxic group as compared with the control group. However, in the HX + CLQ group, there was a statistically significant decrease in the vessel medial wall as compared with the HX group. In the TRPC1‐, TRPC6‐, and CaSR‐immunopositive cell numbers, messenger RNA expressions and biochemical results showed an increase in the HX group, whereas they were decreased in the HX + CLQ group. The inhibitory effect of CLQ on calcium receptors in arterioles was observed in PAH.

中文翻译：

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缺氧诱导的实验性肺动脉高压中氯喹对肺动脉平滑肌细胞TRPC1，TRPC6和CaSR的影响

肺动脉高压（PAH）是一种威胁生命的疾病，其特征是持续的高肺动脉压和血管重塑。已观察到氯喹（CLQ）抑制钙流入。这项研究的目的是研究低氧PAH模型中CLQ对瞬时受体阳离子蛋白（TRPC1和TRPC6）和细胞外钙敏感受体（CaSR）的影响。在这项研究中，使用了8至12周大的雄性Wistar白化病大鼠32只，重200至300 g。对大鼠进行了四组研究，包括正常对照组，n = 8；对照组，n = 8。去甲氧基CLQ（50 mg / kg / 28 d），n = 8；低氧（HX; 10％氧气/ 28 d）对照，n = 8; HX（10％氧气/ 28 d）+ CLQ（50 mg / kg），N =8。采用免疫组织化学方法评估肺动脉内壁厚度，肺小动脉壁，TRPC1，TRPC6和CaSR的表达，聚合酶链反应和酶联免疫吸附测定方法。在实验结束时，与对照组相比，在低氧组中观察到内侧壁厚度的统计学显着增加。然而，在HX + CLQ组中，与HX组相比，血管内侧壁有统计学上的显着下降。在TRPC1，TRPC6和CaSR免疫阳性细胞数量中，信使RNA表达和生化结果显示，HX组增加，而HX + CLQ组下降。在PAH中观察到CLQ对小动脉钙受体的抑制作用。与对照组相比，在低氧组中观察到内侧壁厚度的统计学显着增加。然而，在HX + CLQ组中，与HX组相比，血管内侧壁有统计学上的显着下降。在TRPC1，TRPC6和CaSR免疫阳性细胞数量中，信使RNA表达和生化结果显示，HX组增加，而HX + CLQ组下降。在PAH中观察到CLQ对小动脉钙受体的抑制作用。与对照组相比，在低氧组中观察到内侧壁厚度的统计学显着增加。然而，在HX + CLQ组中，与HX组相比，血管内侧壁有统计学上的显着下降。在TRPC1，TRPC6和CaSR免疫阳性细胞数量中，信使RNA表达和生化结果显示，HX组增加，而HX + CLQ组下降。在PAH中观察到CLQ对小动脉钙受体的抑制作用。信使RNA的表达和生化结果显示，HX组增加，而HX + CLQ组下降。在PAH中观察到CLQ对小动脉钙受体的抑制作用。信使RNA的表达和生化结果显示，HX组增加，而HX + CLQ组下降。在PAH中观察到CLQ对小动脉钙受体的抑制作用。