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Characterization of seminal parameters, sperm morphometry, micromorphology, and ultrastructure in gray brocket deer (Mazama gouazoubira, Fischer, 1814).
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2020-09-21 , DOI: 10.1002/jemt.23590
Duanny Murinelly de Souza Cunha 1 , Mírley Barbosa de Souza 1 , Bruna Farias Brito 1 , Herlon Victor Rodrigues Silva 1 , Leandro Rodrigues Ribeiro 2 , Francisco Antônio Félix Xavier Júnior 1 , Janaina Serra Azul Monteiro Evangelista 1 , Leda Maria Costa Pereira 1 , Dárcio Ítalo Alves Teixeira 1
Affiliation  

Populations of gray brocket deer (Mazama gouazoubira) are declining; yet, knowledge on the reproductive biology of this species remains limited. Therefore, this study aimed to describe morphology, viability, membrane integrity, mitochondrial activity, morphometry, micromorphology, and ultrastructure of the gray brocket deer sperm. Three adult male gray brocket deer were used in the study. Semen collection was performed using electroejaculation. Semen were analyzed by evaluating pH, motilities, vigor, mass movement, volume, concentration, viability, membrane integrity, mitochondrial activity, morphology, and morphometry. Micromorphology and ultrastructure of sperm were analyzed using scanning and transmission electron microscopy (SEM and TEM), respectively. There was no significant difference among males regarding on pH, motilities, vigor, mass movement, volume, concentration, viability. High values for membrane integrity, mitochondrial activity, and normal sperm were observed. The most frequent defects were simple bent tail and bowed midpiece. The head length, and width, midpiece, and tail length were 8.5, 4.4, 11.5, and 41.3 μm, respectively. SEM sperm showed paddle‐shaped heads, with apical ridge and serrated band on the equatorial segment. TEM revealed the nucleus, acrosome, plasma membrane, mitochondria sheath, proximal centrioles, segmented columns, axoneme, outer dense fibers, and fibrous sheath. SEM and TEM showed the presence of some abnormalities. These results are expected to provide baseline values of diverse semen parameters, contributing toward the development of reproductive biotechnologies for gray brocket deer and, other deer species at risk of extinction.

中文翻译:

灰斑鹿的精液参数、精子形态测量学、微形态学和超微结构的表征(Mazama gouazoubira,Fischer,1814)。

灰斑鹿(Mazama gouazoubira )的种群) 正在下降;然而,对该物种生殖生物学的了解仍然有限。因此,本研究旨在描述灰石鹿精子的形态、活力、膜完整性、线粒体活性、形态测量学、微形态学和超微结构。研究中使用了三只成年雄性灰斑鹿。使用电射精进行精液收集。通过评估 pH 值、运动性、活力、质量运动、体积、浓度、活力、膜完整性、线粒体活性、形态学和形态测定法来分析精液。分别使用扫描和透射电子显微镜(SEM 和 TEM)分析精子的显微形态和超微结构。雄性在 pH 值、运动性、活力、质量运动、体积、浓度、活力方面没有显着差异。观察到膜完整性、线粒体活性和正常精子的高值。最常见的缺陷是简单的弯曲尾翼和弓形中间件。头部长度、宽度、中段和尾部长度分别为 8.5、4.4、11.5 和 41.3 μm。SEM精子呈桨状头部,赤道段有尖脊和锯齿带。TEM 显示细胞核、顶体、质膜、线粒体鞘、近端中心粒、分段柱、轴丝、外致密纤维和纤维鞘。SEM和TEM显示存在一些异常。预计这些结果将提供各种精液参数的基线值,有助于开发灰胸鹿和其他濒临灭绝的鹿物种的生殖生物技术。并观察到正常精子。最常见的缺陷是简单的弯曲尾翼和弓形中间件。头部长度、宽度、中段和尾部长度分别为 8.5、4.4、11.5 和 41.3 μm。SEM精子呈桨状头部,赤道段有尖脊和锯齿带。TEM 显示细胞核、顶体、质膜、线粒体鞘、近端中心粒、分段柱、轴丝、外致密纤维和纤维鞘。SEM和TEM显示存在一些异常。预计这些结果将提供各种精液参数的基线值,有助于开发灰胸鹿和其他濒临灭绝的鹿物种的生殖生物技术。并观察到正常精子。最常见的缺陷是简单的弯曲尾翼和弓形中间件。头部长度、宽度、中段和尾部长度分别为 8.5、4.4、11.5 和 41.3 μm。SEM精子呈桨状头部,赤道段有尖脊和锯齿带。TEM 显示细胞核、顶体、质膜、线粒体鞘、近端中心粒、分段柱、轴丝、外致密纤维和纤维鞘。SEM和TEM显示存在一些异常。预计这些结果将提供各种精液参数的基线值,有助于开发灰胸鹿和其他濒临灭绝的鹿物种的生殖生物技术。中段和尾部长度分别为 8.5、4.4、11.5 和 41.3 μm。SEM精子呈桨状头部,赤道段有尖脊和锯齿带。TEM 显示细胞核、顶体、质膜、线粒体鞘、近端中心粒、分段柱、轴丝、外致密纤维和纤维鞘。SEM和TEM显示存在一些异常。预计这些结果将提供各种精液参数的基线值,有助于开发灰胸鹿和其他濒临灭绝的鹿物种的生殖生物技术。中段和尾部长度分别为 8.5、4.4、11.5 和 41.3 μm。SEM精子呈桨状头部,赤道段有尖脊和锯齿带。TEM 显示细胞核、顶体、质膜、线粒体鞘、近端中心粒、分段柱、轴丝、外致密纤维和纤维鞘。SEM和TEM显示存在一些异常。预计这些结果将提供各种精液参数的基线值,有助于开发灰胸鹿和其他濒临灭绝的鹿物种的生殖生物技术。近端中心粒、分段柱、轴丝、外致密纤维和纤维鞘。SEM和TEM显示存在一些异常。预计这些结果将提供各种精液参数的基线值,有助于开发灰胸鹿和其他濒临灭绝的鹿物种的生殖生物技术。近端中心粒、分段柱、轴丝、外致密纤维和纤维鞘。SEM和TEM显示存在一些异常。预计这些结果将提供各种精液参数的基线值,有助于开发灰胸鹿和其他濒临灭绝的鹿物种的生殖生物技术。
更新日期:2020-09-21
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