当前位置: X-MOL 学术Clin. Exp. Immunol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Relationship between natural and infection -induced antibodies in systemic autoimmune diseases (SAD); SLE, SSc and RA.
Clinical & Experimental Immunology ( IF 3.4 ) Pub Date : 2020-09-22 , DOI: 10.1111/cei.13521
K Böröcz 1 , D Simon 1 , S Erdő-Bonyár 1, 2 , K T Kovács 2 , É Tuba 2 , L Czirják 2 , P Németh 1 , T Berki 1
Affiliation  

Infection or vaccine‐induced T cell‐dependent immune response and the subsequent high‐affinity neutralizing antibody production have been extensively studied, while the connection between natural autoantibodies (nAAbs) and disease‐specific antibodies has not been thoroughly investigated. Our goal was to find the relationship between immunoglobulin (Ig)M and IgG isotype nAAbs and infection or vaccine‐induced and disease‐related autoantibody levels in systemic autoimmune diseases (SAD). A previously described indirect enzyme‐linked immunosorbent assay (ELISA) test was used for detection of IgM/IgG nAAbs against citrate synthase (anti‐CS) and F4 fragment (anti‐F4) of DNA topoisomerase I in 374 SAD samples, with a special focus on systemic lupus erythematosus (SLE) (n = 92), rheumatoid arthritis (n = 73) and systemic sclerosis (n = 157) disease groups. Anti‐measles IgG and anti‐dsDNA IgG/IgM autoantibodies were measured using commercial and in‐house indirect ELISA tests. In all SAD groups the anti‐measles IgG‐seropositive cases showed significantly higher anti‐CS IgG titers (P = 0·011). In anti‐dsDNA IgG‐positive SLE patients, we detected significantly higher levels of anti‐CS and anti‐F4 IgG nAAbs (P = 0·001 and < 0·001, respectively). Additionally, we found increased levels of IgM isotypes of anti‐CS and anti‐F4 nAAbs in anti‐dsDNA IgM‐positive SLE patients (P = 0·002 and 0·016, respectively). The association between IgG isotypes of pathogen‐ or autoimmune disease‐related antibodies and the IgG nAAbs may underscore the immune response‐inducible nature of the diseases investigated. The relationship between protective anti‐dsDNA IgM and the IgM isotype of anti‐F4 and anti‐CS may provide immunoserological evidence for the beneficial roles of nAAbs in SLE patients.

中文翻译:

系统性自身免疫性疾病(SAD)中天然抗体和感染诱导抗体之间的关系;SLE、SSc 和 RA。

感染或疫苗诱导的 T 细胞依赖性免疫反应以及随后的高亲和力中和抗体的产生已被广泛研究,而天然自身抗体 (nAAb) 和疾病特异性抗体之间的联系尚未得到彻底研究。我们的目标是找出系统性自身免疫性疾病 (SAD) 中免疫球蛋白 (Ig)M 和 IgG 同型 nAAb 与感染或疫苗诱导的以及疾病相关的自身抗体水平之间的关系。先前描述的间接酶联免疫吸附测定 (ELISA) 测试用于检测 374 个 SAD 样本中针对柠檬酸合酶(抗 CS)和 DNA 拓扑异构酶 I 的 F4 片段(抗 F4)的 IgM/IgG nAAb,重点关注系统性红斑狼疮 (SLE) ( n  = 92)、类风湿性关节炎 ( n  = 73) 和系统性硬化症 ( n  = 157) 疾病群体。使用商业和内部间接 ELISA 测试测量抗麻疹 IgG 和抗 dsDNA IgG/IgM 自身抗体。在所有 SAD 组中,抗麻疹 IgG 血清阳性病例均表现出显着较高的抗 CS IgG 滴度 ( P  = 0·011)。在抗 dsDNA IgG 阳性 SLE 患者中,我们检测到抗 CS 和抗 F4 IgG nAAb 水平显着升高( 分别为P = 0·001 和 < 0·001)。此外,我们发现抗 dsDNA IgM 阳性 SLE 患者中抗 CS 和抗 F4 nAAb 的 IgM 同种型水平升高(分别为P  = 0·002 和 0·016)。病原体或自身免疫性疾病相关抗体的 IgG 同种型与 IgG nAAb 之间的关联可能强调所研究疾病的免疫反应诱导性质。保护性抗 dsDNA IgM 与抗 F4 和抗 CS 的 IgM 同种型之间的关系可能为 nAAb 在 SLE 患者中的有益作用提供免疫血清学证据。
更新日期:2020-09-22
down
wechat
bug