Recent environmental and health concerns have been pushing the society for the consumption of natural and sustainable products, as for example, the use of natural colorants as additives to impart color. Talaromyces amestolkiae are natural producers of red colorants at both extracellular and cell-bond state, so are very important for the dye industry. T. amestolkiae cells are constituted by a complex and rigid cell wall structure, which contain a major fraction of the total colorants content. The use of environmentally friendly, biocompatible and high-efficiency cell disruption procedures is thus critical for the recovery of the intracellular colorants. An alternative approach using imidazolium-based ionic liquids (ILs) was applied to recover the intracellular colorants from T. amestolkiae fungi. First, the cell disruption ability of a series 1-alkyl-3-methylimidazolium chloride ILs ([C_nmim]Cl) aqueous solutions was determined, assessing both the effect of increasing cationic alkyl chain length (n = 1, 2, 4, 6, 8 or 10) and IL concentration (30% and 45% w/v). [C₄mim]Cl showed the highest extraction ability to recover red, orange and yellow colorants. In the second part, the extraction ability of [C₄mim]Cl (at 45% w/v in water) was improved, by combining the IL solution chemical treatment with a mechanical (ultrasound-temperature-assisted) cell disrupting method. At this point, the incubation time (at 60 °C and 150 rpm) after sonication, number sonication pulses (5 min ON) and re-extraction effects were screened as parameters for increasing the cell disruption. The mechanical-assisted method allowed an increase of almost 3-fold in the extractive performance of [C₄mim]Cl solution, achieving a maximum recovery of 76.07 UA_490nm/mL of red colorants. The results demonstrate the potential of [C₄mim]Cl solution as chemical cell-disrupting aid in ultrasound-temperature-based methods to improve the recovery of intracellular colorants from complex filamentous fungi cells.

最近对环境和健康的关注已推动社会消费天然和可持续产品，例如，使用天然着色剂作为添加剂来赋予颜色。伞形拟南芥（Talaromyces amestolkiae）是细胞外和细胞结合状态的红色着色剂的天然生产者，因此对染料工业非常重要。T. amestolkiae细胞由复杂且刚性的细胞壁结构组成，其中包含总着色剂含量的大部分。因此，使用环境友好，生物相容和高效的细胞破碎程序对于细胞内着色剂的回收至关重要。使用基于咪唑鎓离子液体（IL）的另一种方法可从中回收细胞内着色剂T.amestolkiae真菌。首先，确定一系列1-烷基-3-甲基咪唑鎓氯化物ILs（[C _n mim] Cl）水溶液的细胞破坏能力，同时评估增加阳离子烷基链长的影响（n  = 1、2、4， 6、8或10）和IL浓度（30％和45％w / v）。[C ₄ mim] Cl表现出最高的回收红色，橙色和黄色着色剂的能力。在第二部分中，[C ₄通过将IL溶液化学处理与机械（超声温度辅助）细胞破碎方法相结合，改善了mim] Cl（在水中含量为45％w / v时）。此时，筛选超声处理后的孵育时间（60°C和150 rpm），超声处理次数（ON 5分钟）和再提取效果作为增加细胞分裂的参数。机械辅助方法使[C ₄ mim] Cl溶液的萃取性能提高了近3倍，最大回收率为76.07 UA _490nm / mL红色染料。结果证明了[C ₄mim] Cl溶液在基于超声温度的方法中可作为化学破坏细胞的辅助手段，以改善复杂丝状真菌细胞中细胞内着色剂的回收率。