The development of efficient and clean CO₂ capture technologies is a topic of utmost importance in the global environmental agenda. In this work, a wide range of analytical procedures (¹H and ¹³C NMR, TGA, DSC, FT-IR, viscosity, density, refractive index, fluorescence anisotropy, UV–Vis) was used to investigate the properties of deep eutectic solvent (DES) composed of choline chloride and levulinic acid (ChCl:LA), conditioned to four different water activity (a_w) values. Also, the impact of using the enzyme carbonic anhydrase (CA), known for its ability to reversibly convert CO₂ into bicarbonate, was assessed on DES properties. The ChCl:LA-water mixtures showed to be present as a cohesive cluster up to a_w = 0.753 and, above that, in a hydrated form with a free water layer that could crystalize at negative temperatures (-40 to −45 °C), suggesting anti-freezing properties of the solvent. DES were then supported in polymeric hydrophilic membranes. Based on CO₂ and N₂ permeabilities through these membranes, the one that contained ChCl:LA at a_w = 0.216 was further studied regarding its long-term stability. This supported liquid membrane showed a consistent behavior for up to 30 repeated cycles and a stable operating behavior for transmembrane pressure differences in the range of 0.50–1.25 bar. These findings evidence the stability of supported DES membranes and the potential for CO₂ capture.

高效，清洁的CO ₂捕集技术的发展是全球环境议程中极为重要的主题。在这项工作中，广泛的分析程序（¹ H和¹³ C NMR，TGA，DSC，FT-IR，粘度，密度，折射率，荧光各向异性，UV-Vis）用于研究深共熔溶剂的性质。 （DES）的氯化胆碱和乙酰丙酸构成的（CHCL：LA），调节至四个不同的水活度（一_瓦特）值。此外，还评估了使用碳酸酐酶（CA）的影响（对DES性能的影响），该酶以可逆方式将CO ₂转化为碳酸氢根而闻名。ChCl：LA-水的混合物显示为聚集成团状存在_w  = 0.753，并且在此之上呈水合形式，带有游离水层，该游离水层可在负温度（-40至-45°C）下结晶，表明溶剂的抗冻性能。然后将DES负载在聚合物亲水膜中。基于CO ₂和N ₂通过这些膜的渗透性， 进一步研究了其在_w = 0.216时包含ChCl：LA的长期稳定性。这种支撑的液膜在多达30个重复循环中表现出一致的行为，并且跨膜压差在0.50–1.25 bar范围内具有稳定的操作行为。这些发现证明了支持的DES膜的稳定性和捕获CO ₂的潜力。