Plants utilize different mechanisms to respond and adapt to continuously changing environmental factors. Receptor-like protein kinases (RLKs) comprise one of the largest families of plant transmembrane signaling proteins, which play critical and diverse roles in plant growth, development, and stress response. Here, we identified the necrotic dwarf (ndw) mutant introgression line (IL) 6-2, which demonstrated stunting, leaf curl, and progressive necrosis at low temperatures. Based on map-based cloning and transgenic analysis, we determined that the phenotype of ndw mutant is caused by decreased expression of NDW, which encodes an RLK. NDW is a plasma membrane and cytoplasmic located protein. Overexpression of NDW can restore both of the semi-dwarf and necrotic phenotype in IL6-2 at low temperatures, further we found that NDW could significantly reduce susceptibility to Botrytis cinerea. On the contrary, knockdown NDW in M82 plants could increase the sensitivity to B. cinerea. Furthermore, transcriptional expression analysis showed that NDW affects the expression of genes related to the abscisic acid (ABA) signaling pathway. Taken together, these results indicate that NDW plays an important role in regulating plant growth, cold tolerance and mitigating susceptibility to Botrytis cinerea.

中文翻译：

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NDW 编码受体样蛋白激酶，调节植物生长、耐寒性和对番茄灰葡萄孢菌的易感性

植物利用不同的机制来响应和适应不断变化的环境因素。受体样蛋白激酶 (RLK) 是最大的植物跨膜信号蛋白家族之一，在植物生长、发育和胁迫反应中发挥着重要且多样的作用。在这里，我们鉴定了坏死性矮化 (ndw) 突变体基因渗入系 (IL) 6-2，其在低温下表现出发育迟缓、叶片卷曲和进行性坏死。基于基于图谱的克隆和转基因分析，我们确定 ndw 突变体的表型是由编码 RLK 的 NDW 表达降低引起的。NDW 是位于质膜和细胞质中的蛋白质。NDW 的过表达可以在低温下恢复 IL6-2 的半矮化和坏死表型，我们进一步发现 NDW 可以显着降低对灰葡萄孢菌的易感性。相反，在 M82 植物中敲低 NDW 可以增加对 B. cinerea 的敏感性。此外，转录表达分析表明，NDW 影响与脱落酸 (ABA) 信号通路相关的基因的表达。总之，这些结果表明 NDW 在调节植物生长、耐寒和减轻对灰葡萄孢的易感性方面起着重要作用。