Palladium (Pd) is commonly used as a catalyst for automobiles and electronic devices, and a reliable source of Pd is required for continued commercial applications. Biomineralization has attracted attention as an inexpensive and eco-friendly recycling approach for a continued supply of Pd. Escherichia coli is one of the best hosts for collecting Pd because it grows rapidly and requires an inexpensive minimal medium. Although E. coli can reduce Pd ions, the mechanism of reduction has not been thoroughly investigated. In this study, we investigated the genes involved in the reduction of Pd ions in E. coli. A gene responsible for the reduction of Pd ions was identified from approximately 4000 genes, other than essential genes, by using the single-gene-knockout library. The rate of reducing Pd ions by E. coli cells was evaluated. Among the investigated single-gene-knockout strains, 7 strains including the gene related to membrane transport, transcriptional regulation, and metabolic enzyme promote the reduction of Pd ions, and 73 strains including the genes related to formate metabolism and molybdopterin synthesis repress the reduction of Pd ions. Our results may provide a new perspective for the improvement of the bioreduction of minor metals.

钯 (Pd) 通常用作汽车和电子设备的催化剂，持续的商业应用需要可靠的 Pd 来源。生物矿化作为一种​​廉价且环保的回收方法，可用于持续供应 Pd，因此引起了人们的关注。大肠杆菌是收集 Pd 的最佳宿主之一，因为它生长迅速并且需要廉价的基本培养基。尽管大肠杆菌可以还原 Pd 离子，但还原机制尚未得到彻底研究。在这项研究中，我们研究了参与还原大肠杆菌中 Pd 离子的基因. 通过使用单基因敲除文库，从除必需基因以外的大约 4000 个基因中鉴定了负责减少 Pd 离子的基因。评估了大肠杆菌细胞减少 Pd 离子的速率。在研究的单基因敲除菌株中，与膜转运、转录调控和代谢酶相关的基因7株促进Pd离子的减少，与甲酸代谢和钼蝶呤合成相关的基因73株抑制Pd离子的减少。钯离子。我们的研究结果可能为改善微量金属的生物还原提供新的视角。