当前位置: X-MOL 学术J. Biotechnol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Synthesis of α-hydroxy ketones and vicinal diols with the Bacillus licheniformis DSM 13T butane-2,3-diol dehydrogenase
Journal of Biotechnology ( IF 4.1 ) Pub Date : 2020-09-22 , DOI: 10.1016/j.jbiotec.2020.09.016
Lukas Muschallik 1 , Carina Ronja Kipp 1 , Inga Recker 1 , Johannes Bongaerts 1 , Martina Pohl 2 , Melanie Gellissen 1 , Michael J Schöning 1 , Thorsten Selmer 1 , Petra Siegert 1
Affiliation  

The enantioselective synthesis of α-hydroxy ketones and vicinal diols is an intriguing field because of the broad applicability of these molecules. Although, butandiol dehydrogenases are known to play a key role in the production of 2,3-butandiol, their potential as biocatalysts is still not well studied. Here, we investigate the biocatalytic properties of the meso-butanediol dehydrogenase from Bacillus licheniformis DSM 13T (BlBDH). The encoding gene was cloned with an N-terminal StrepII-tag and recombinantly overexpressed in E. coli. BlBDH is highly active towards several non-physiological diketones and α-hydroxyketones with varying aliphatic chain lengths or even containing phenyl moieties. By adjusting the reaction parameters in biotransformations the formation of either the α-hydroxyketone intermediate or the diol can be controlled.



中文翻译:

用地衣芽孢杆菌 DSM 13T 丁烷-2,3-二醇脱氢酶合成 α-羟基酮和邻二醇

由于这些分子的广泛适用性,α-羟基酮和邻二醇的对映选择性合成是一个有趣的领域。尽管已知丁二醇脱氢酶在 2,3-丁二醇的生产中起关键作用,但它们作为生物催化剂的潜力仍未得到充分研究。在这里,我们研究了来自地衣芽孢杆菌DSM 13 T (BlBDH)的内消旋丁二醇脱氢酶的生物催化特性。编码基因用 N 端 StrepII 标签克隆,并在大肠杆菌中重组过表达. BlBDH 对几种具有不同脂肪族链长度或什至含有苯基部分的非生理双酮和 α-羟基酮具有高度活性。通过调整生物转化中的反应参数,可以控制 α-羟基酮中间体或二醇的形成。

更新日期:2020-10-04
down
wechat
bug