Phytase inclusion provides broiler production advantages such as improved growth performance and better nutrient digestibility. These effects become heightened in response to more complete degradation of IP6 and its lower esters to prevent chelation of nutrients. Therefore, strategies for the complete removal of the total phytate pool are being investigated to maximize the benefits previously mentioned. The unique biochemical properties amongst commercial phytases may provide an opportunity for a more coordinated and efficacious approach to dephosphorylation if fed together. Therefore, 3 phytases (A, B, and C) were evaluated for potential synergistic action in breaking down on phytate using phytase inclusion strategies (PIS; A, B, C, AB, AC, BC, or ABC) at either 120 or 240 FTU/kg using 0–14 d chick performance, as well as tibia ash and ileal measurements (digestibility and IP esters). Positive and negative control (PC; NC) diets were formulated to contain 0.8% Ca and 0.4% nonphytate P (nPP) and 0.4% Ca and 0.20% nPP, respectively. Feeding NC + ABC at 240 FTU/kg led to improved BW gain and tibia ash percentage. Reduction of IP6/IP5 and increased IP isomers were found with NC + PIS treatments vs. PC, with benefits for PIS treatments at 240 FTU/kg. Supplementation of NC + B, C, and BC at 240 FTU/kg increased IP4 compared with PC. Digestibility data were inconsistent; however, improvements were demonstrated with feeding NC + B and BC at 240 FTU/kg. These data demonstrate a potential synergy with NC + ABC at 240 FTU/kg. Further research should investigate the effects of PIS with various diet specifications and at higher phytase activity levels.

植酸酶的掺入提供了肉鸡生产的优势，如改善的生长性能和更好的养分消化率。响应IP6及其低级酯的更完全降解以防止营养物质的螯合，这些作用会增强。因此，正在研究完全去除总植酸池的策略，以使前述收益最大化。如果将它们一起食用，商业植酸酶中独特的生化特性可能会为更协调有效的脱磷酸方法提供机会。因此，使用植酸酶包涵体策略（PIS，对3种植酸酶（A，B和C）在分解植酸中的潜在协同作用进行了评估; A，B，C，AB，AC，BC或ABC），使用0–14 d雏鸡性能，以及120到240 FTU / kg，以及胫骨灰分和回肠测量值（消化率和IP酯）。阳性和阴性对照（PC ; NC）日粮的配方均包含0.8％的钙和0.4％的非植酸磷（nPP）），0.4％的Ca和0.20％的nPP。以240 FTU / kg的剂量饲喂NC + ABC可提高体重增加和胫骨灰分。与PC相比，NC + PIS处理降低了IP6 / IP5，IP异构体增加，以240 FTU / kg进行PIS处理具有优势。与PC相比，以240 FTU / kg的剂量添加NC + B，C和BC可增加IP4。消化率数据不一致；然而，饲喂NC + B和BC的剂量为240 FTU / kg时，情况有所改善。这些数据证明了在240 FTU / kg下与NC + ABC的潜在协同作用。进一步的研究应研究各种饮食规格和更高植酸酶活性水平下PIS的影响。