Herein, we have successfully synthesized a novel N-Succinyl chitosan/gold nanocomposite (N-SuC/Au NC) using N-SuC and gold(III) chloride, and investigated the biocompatibility and antifungal activity. The synthesized N-SuC/Au NC was characterized by UV–visible spectroscopy, X-ray diffraction, field emission scanning electron microscope, and inductively coupled plasma atomic emission spectroscopy. The N-SuC/Au NC exhibited a strong inhibition effect towards pathogenic Candida albicans. Morphological analysis revealed the destruction of C. albicans cell membrane due to N-SuC/Au NC treatment. The in vitro and in vivo toxicity of N-SuC/Au NC was analyzed with HEK293T mammalian cells and zebrafish larvae, respectively. The synthesized N-SuC/Au NC demonstrated no cytotoxicity towards HEK293T cells up to 1200 μg/mL concentration. The survival rate of the zebrafish larvae at 120 hpf, was found as 100% up to 1200 μg/mL of N-SuC/Au NC exposure. The in vivo studies further confirmed the inhibitory effects of N-SuC/Au NC on the formation of C. albicans hyphae in infected zebrafish muscle tissue.

在这里，我们已经成功地使用N -SuC和氯化金（III）合成了新型的N-琥珀酰壳聚糖/金纳米复合材料（N -SuC / Au NC），并研究了其生物相容性和抗真菌活性。合成的N -SuC / Au NC具有紫外可见光谱，X射线衍射，场发射扫描电子显微镜和电感耦合等离子体原子发射光谱的特征。所述Ñ -Suc / Au的NC显示出朝向致病很强的抑制作用白色念珠菌。形态分析显示C的破坏。N -SuC / Au NC处理导致的白色细胞膜。的用HEK293T哺乳动物细胞和斑马鱼幼虫分别分析N- SuC / Au NC的体外和体内毒性。合成的N -SuC / Au NC对浓度高达1200μg/ mL的HEK293T细胞无细胞毒性。斑马鱼幼虫在120 hpf的存活率最高为1200μg/ mL N -SuC / Au NC暴露量为100％。的体内研究进一步证实的抑制作用Ñ -Suc / Au的NC上的形成Ç。被感染的斑马鱼肌肉组织中的白色菌丝。