Panax notoginseng is an important medicinal plant that belongs to the Araliceae family. In this study, a stable and high-efficiency system for the proliferation of P. notoginseng adventitious roots was established via two steps, and their growth and accumulation of three ginsenosides (Rg1, Re and Rb1) was analyzed in suspension culture. Adventitious roots were firstly induced from the cotyledons of P. notoginseng seeds, in 1/2 Schenk and Hildebrandt (SH) medium with 2.0 mg L⁻¹ indole-3-butyric acid (IBA) and 30 g L⁻¹ sucrose. Using these adventitious roots as explants, the influence of 1/2 SH and 1/2 Murashige and Skoog (MS) medium without NH₄NO₃ (1/2MS-N), and IBA (0–4.0 mg L⁻¹) and naphthalene acetic acid (NAA) (0–2.0 mg L⁻¹) on the induction of new adventitious roots was investigated. Culture conditions of 1/2 SH with 2.0 mg L⁻¹ IBA and 2.0 mg L⁻¹ NAA were optimal for the formation of new adventitious roots, and led to an induction rate of 100 % and 12.62 new adventitious roots on average for each explant after 3 weeks of culture. In culture conditions of 1/2MS-N with 2.0 mg L⁻¹ IBA and 50 g L⁻¹ sucrose, induced adventitious roots rapidly elongated to a mean length of 14.23 mm within 3 weeks, demonstrating that a two-step process of adventitious root regeneration involving steps of initiation and elongation was most effective. Suspension cultures of adventitious roots were analyzed to evaluate the effects of culture cycle time, concentrations of sucrose and IBA and amount of inoculation on adventitious roots. After 7 weeks, the total yield of three ginsenosides (11.13 mg 100 mL^-1) was highest in root cultures with 1/2 MS-N, 30 g L⁻¹ sucrose, 4.0 mg L⁻¹ IBA, and 3.5 g 100 mL⁻¹ root inoculation culture. The highest total concentration of ginsenosides (18.58 mg g⁻¹ DW) was obtained from adventitious roots cultured from 3.0 g 100 mL⁻¹ inoculation material and the concentration of ginsenoside Re (8.05 mg g⁻¹ DW) was 1.8-fold higher than that in 3-year-old roots cultivated in the field (4.44 mg g⁻¹ DW). The establishment of this culture system will be beneficial for the scale-up culture of adventitious roots and ginsenoside production in P. notoginseng.

三七是一种重要的药用植物，属于伞形科。本研究通过两个步骤建立了稳定的高效三七不定根增殖系统，并在悬浮培养中分析了三种人参皂苷（Rg1，Re和Rb1）的生长和积累。不定根首先是由三叶参种子的子叶在含有2.0 mg L ^-1吲哚-3-丁酸（IBA）和30 g L ^-1蔗糖的1/2 Schenk和Hildebrandt（SH）培养基中诱导的。使用这些不定根作为外植体，可以得到没有NH ₄ NO ₃的1/2 SH和1/2 Murashige和Skoog（MS）培养基的影响（1 / 2MS-N）和IBA（0–4.0 mg  L ^-1）和萘乙酸（NAA）（0–2.0 mg  L ^-1）对诱导新不定根的影响进行了研究。1/2 SH与2.0 mg L ^-1 IBA和2.0 mg L ^-1 NAA的培养条件最适合形成新的不定根，并且每个外植体的平均诱导率分别为100％和12.62个新不定根培养3周后。在具有2.0 mg L ^-1 IBA和50 g L ^-1的1 / 2MS-N培养条件下蔗糖诱导的不定根在3周内迅速拉长至平均长度14.23 mm，这表明不定根再生的两步过程（包括起始和延长步骤）最为有效。分析不定根的悬浮培养，以评估培养周期，蔗糖和IBA的浓度以及接种量对不定根的影响。7周后，三种人参皂苷（11.13 mg 100 mL ^-1）的总产量在具有1/2 MS-N，30 g L ^-1蔗糖，4.0 mg L ^-1 IBA和3.5 g 100 mL的根培养物中最高。^-1根接种培养物。人参皂甙的最高总浓度（18.58 mg g ^-1DW）是从3.0 g 100 mL ^-1接种材料培养的不定根中获得的，人参皂甙Re（8.05 mg g ^-1 DW）的浓度比田间种植的3岁根的浓度高1.8倍（ 4.44 mg g ^-1 DW）。该培养体系的建立将有利于扩大三七不定根和人参皂甙的生产。