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Biomimetic remineralization of artificial caries dentin lesion using Ca/P-PILP.
Dental Materials ( IF 4.6 ) Pub Date : 2020-09-22 , DOI: 10.1016/j.dental.2020.08.017
Ruitian Chen 1 , Runze Jin 1 , Xin Li 2 , Xiaolin Fang 1 , Ding Yuan 1 , Zhuo Chen 3 , Shasha Yao 4 , Ruikang Tang 4 , Zhi Chen 1
Affiliation  

Objective

To assess the ultrastructural change of demineralized dentin collagen during calcium phosphate polymer-induced liquid precursor (Ca/P-PILP) mediated remineralization process and to evaluate the biomimetic remineralization potential of high concentration Ca/P-PILP at demineralized artificial caries dentin lesion, additionally to investigate the bond interfacial integrity as well as the bonding strength of the biomimetic remineralized artificial caries dentin lesion.

Methods

Demineralized dentin collagen of 5 μm thick was biomimetically remineralized with low, medium concentration Ca/P-PILP for 10 days and high concentration Ca/P-PILP for 10, 15, 20 days. Artificial caries dentin lesion at a thickness of 150 ± 50 μm was biomimetically remineralized with high concentration Ca/P-PILP for 20 days. The biomimetic remineralization of demineralized dentin collagen was observed by scanning electron microscopy (SEM). The biomimetic remineralization intensity and depth of artificial caries dentin lesion was assessed by Electron Probe Micro Analyzer (EPMA). The bonding interfacial integrity between remineralized artificial caries dentin and composite resin was observed by Swept-source optical coherence tomography (SS-OCT) and the bonding strength of remineralized artificial caries dentin was evaluated by micro-tensile bond strength analysis (μTBS).

Results

Solely PAA-PASP solution and solely saturated Ca/P solution can’t achieve dentin collagen remineralization. Increased concentration of Ca/P-PILP and prolonged remineralization time can enhance the biomimetic remineralization intensity of demineralized dentin collagen. After treating with high concentration Ca/P-PILP, a 150 ± 50 μm thick layer of demineralized artificial caries dentin lesion was not fully remineralized, and the biomimetic remineralization intensity reached up to 88.0%. Furthermore, a better bonding interfacial integrity with less microgap and increased bond strength at both baseline level and aging level were observed when artificial caries dentin lesion was biomimetically remineralized with high concentration Ca/P-PILP.

Significance

Biomimetic remineralization of demineralized caries dentin lesion promotes its clinical properties for resin composited adhesive restoration.



中文翻译:

使用Ca / P-PILP仿造龋齿牙本质病灶的仿生矿化作用。

目的

评估脱钙牙本质胶原蛋白在磷酸钙聚合物诱导的液体前体(Ca / P-PILP)介导的再矿化过程中的超微结构变化,并评估高浓度Ca / P-PILP在脱钙人工龋牙本质病灶上的仿生再矿化潜力。研究仿生再矿化人工龋牙本质病灶的粘结界面完整性和粘结强度。

方法

将5μm厚的脱矿质牙本质胶原通过低,中等浓度的Ca / P-PILP和高浓度Ca / P-PILP的仿生矿化10天,15天,20天。用高浓度Ca / P-PILP仿生矿化150±50μm厚度的人工龋齿牙本质病变20天。通过扫描电子显微镜(SEM)观察到脱矿的牙本质胶原的仿生再矿化。通过电子探针显微分析仪(EPMA)评估了人工龋齿牙本质病变的仿生再矿化强度和深度。

结果

仅PAA-PASP溶液和单独的饱和Ca / P溶液无法实现牙本质胶原的再矿化。Ca / P-PILP浓度的增加和再矿化时间的延长可以增强脱矿牙本质胶原的仿生再矿化强度。用高浓度Ca / P-PILP处理后,一层150±50μm厚的脱矿质人工龋牙本质病灶未完全矿化,仿生矿化强度达到88.0%。此外,当使用高浓度Ca / P-PILP仿生化人工龋牙本质病损时,在基线水平和老化水平下均观察到更好的结合界面完整性,且微间隙较小,并且结合强度增加。

意义

脱钙龋齿牙本质病灶的仿生再矿化促进其用于树脂复合粘合剂修复的临床特性。

更新日期:2020-10-30
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