Abstract In this study we evaluated the productivity of an outdoor cultivation system based in secondary attachment discs (SADs) of Chondracanthus chamissoi as propagation strategy to produce and maintain a permanent thalli stock. For that, re-attachment of fronds, survival of SADs, growth rate of new erect axes, coverage and productivity were evaluated in three different artificial substrates: fiberglass plates, ceramic plates and PVC pipes. C. chamissoi SADs were formed in all three substrates, and they developed new erect axes reaching >6 mm length after 12 weeks. Ceramic and fiberglass plates were the substrates with the best performance. Nonetheless, the cultivation system using fiberglass was the most suitable one, since it presented a higher survival of the SADs and a better use of tank space, improving the amount of seaweeds growing inside the tanks. Based on the results, the estimated cultivation using fiberglass plates would allow to produce 31,993 SADs on a 3m3 tank after 90 days. This technology would allow: (i) to produce thalli to be used as propagules for seaweed cultivation based on vegetative propagation, since nowadays this depends of thalli from natural populations; (ii) to propagate and maintain a thalli stock with desirable commercial traits, providing an better biomass quality, (iii) to grow and maintain thalli in the same tank from diverse localities and/or reproductive phase; optimizing resources and space, which currently is not feasible using free-floating systems, due to the difficulty to recognize isomorphic haploid and diploid phases, and (iv) to adopt this strategy in other red seaweeds of commercial interest, such as the genus Chondracanthus, Gigartina and Solieria.

中文翻译：

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食用海藻 Chondracanthus chamissoi (Rhodophyta, Gigartinales) 的次级附着盘：建立永久菌群

摘要 在这项研究中，我们评估了基于 Chondracanthus chamissoi 的次生附着盘 (SAD) 的户外栽培系统的生产力，该系统作为繁殖策略来生产和维持永久的菌体种群。为此，在三种不同的人造基材：玻璃纤维板、陶瓷板和 PVC 管中评估了叶的重新附着、SAD 的存活、新直立轴的生长速度、覆盖率和生产率。C. chamissoi SADs 在所有三种基质中都形成，并且它们在 12 周后发展出新的直立轴，长度超过 6 mm。陶瓷和玻璃纤维板是性能最好的基材。尽管如此，使用玻璃纤维的培养系统是最合适的，因为它提供了更高的 SAD 存活率和更好地利用水箱空间，提高水箱内生长的海藻数量。根据结果​​，使用玻璃纤维板进行的估计培养将允许在 90 天后在 3 立方米的水箱中产生 31,993 SAD。该技术将允许： (i) 生产用作基于营养繁殖的海藻养殖繁殖体的菌体，因为现在这取决于来自自然种群的菌体；(ii) 繁殖和维持具有理想商业特性的菌体种群，提供更好的生物质质量， (iii) 在同一水池中生长和维持来自不同地点和/或繁殖阶段的菌体；优化资源和空间，目前使用自由浮动系统是不可行的，因为难以识别同构单倍体和二倍体相，以及 (iv) 在其他具有商业利益的红海藻中采用这种策略，