Abstract The prokaryotic community in the pecan tree (Carya illinoinensis) rhizospheric soil with (clo) and without (non-clo) clover coverage exposed to copper oxide nanoparticles (CuO-NP) was evaluated by 16S rRNA amplicon sequencing. Treatments were applied with CuO-NP at concentrations of 0, 10, 100, and 1000 mg kg−1 soil and maintained for 45 days in microcosms. The analyses indicated that the prokaryotic microbial community of the soils consisted mainly of the phyla Thaumarchaeota, Proteobacteria, Actinobacteria, Bacteroidetes, Acidobacteria and Firmicutes (>80%). In terms of diversity, slight differences were observed between the control groups and their respective treatments and among treatments in both clo and non-clo soils. The community structure analyses showed the orders Caulobacterales, Burkholderiales, Xanthomonadales and Clostridiales significantly abundant (P

中文翻译：

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氧化铜纳米颗粒轻微影响山核桃（Carya illinoinensis）根际土壤中原核生物群落的多样性和代谢特征

摘要 通过 16S rRNA 扩增子测序评估了暴露于氧化铜纳米粒子 (CuO-NP) 的 (clo) 和没有 (non-clo) 三叶草覆盖的山核桃 (Carya illinoinensis) 根际土壤中的原核生物群落。使用浓度为 0、10、100 和 1000 mg kg-1 土壤的 CuO-NP 进行处理，并在微观世界中维持 45 天。分析表明土壤的原核微生物群落主要由奇古菌门、变形菌门、放线菌门、拟杆菌门、酸杆菌门和厚壁菌门组成（>80%）。在多样性方面，对照组和它们各自的处理之间以及在clo和非clo土壤中的处理之间都观察到了细微的差异。群落结构分析显示，目Caulobacterales、Burkholderiales、