Abstract

The effects of some hydrolytic enzymes from marine organisms on the formation and destruction of bacterial biofilms have been studied. As the results show, the presence of α-D-galactosidase from the marine bacterium Pseudoalteromonas sp. KMM 701 stimulates the growth of biofilms formed by various species of marine bacteria, whereas the formation of biofilms by Bacillus subtilis and Yersinia pseudotuberculosis is inhibited by this enzyme. Treatment with α-galactosidase causes destruction of 5 to 35% of a mature biofilm of various bacterial species. Phosphodiesterase and alkaline phosphatase from the marine bacterium Cobetia amphilecti KMM 296 have an inhibitory effect on the biofilm formation by marine strains of Bacillus licheniformis, B. aegricola, and B. berkelogi, and also degrade already formed biofilms of these bacilli and Yersinia. The crab hepatopancreas DNase inhibits the biofilm formation by Y. pseudotuberculosis and B. subtilis by partially degrading a mature biofilm.

中文翻译：

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来自海洋生物的水解酶作为生物膜形成的抑制剂

摘要

已经研究了一些来自海洋生物的水解酶对细菌生物膜形成和破坏的影响。结果表明，海洋细菌Pseudoalteromonas sp。中存在α-D-半乳糖苷酶。KMM 701刺激各种海洋细菌形成的生物膜的生长，而枯草芽孢杆菌和假结核耶尔森氏菌的生物膜的形成却被该酶抑制。用α-半乳糖苷酶处理会破坏各种细菌种类的成熟生物膜的5％至35％。来自海洋细菌Cobetia amphilecti KMM 296的磷酸二酯酶和碱性磷酸酶对海洋菌株的生物膜形成具有抑制作用。地衣芽孢杆菌，B. aegricola和B. berkelogi，并降解这些细菌和耶尔森氏菌已经形成的生物膜。螃蟹肝胰腺DNase通过部分降解成熟的生物膜来抑制假结核耶尔森氏菌和枯草芽孢杆菌的生物膜形成。