The present study reveals the effect of selenium (Se) on different storage proteins accumulation and protein-bound Se in developing wheat grains. A greenhouse experiment was conducted on wheat grown in alkaline sandy loam soil treated with selenate-Se and selenite-Se ranging from 0 to 4 mg kg−1. Selenium content increased significantly in various Se-treated plant tissues as compared to control at maturity. Total soluble proteins and different protein fractions, viz. albumin, globulins, prolamins and glutelins, decreased significantly in Se-treated wheat grains as compared to control. The amount of Se with reference to protein fraction was highest in the glutelin fraction, followed by prolamin, albumin and globulins. Electrophoretic analysis followed by densitometry revealed variations in the presence of polypeptide bands in different protein fractions at maturity. Fourier transform infra red spectra analysis revealed more flexibility and unfolding of structures with higher selenite dosage. The excess binding of Se to protein or amino acid may replace sulfhydryl groups of proteins, thus causing deleterious effects on normal protein form. However, higher concentration of protein-bound Se can be exploited for the fortification of Se-deficient foods in preparation of Se-enriched diets and various health supplements for populations deficient in Se.

中文翻译：

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硒影响小麦籽粒发育过程中蛋白质积累及其在不同蛋白质组分中的分布

本研究揭示了硒 (Se) 对发育中的小麦籽粒中不同贮藏蛋白积累和蛋白质结合硒的影响。对生长在碱性砂壤土中的小麦进行了温室试验，该土壤用硒酸盐-Se 和亚硒酸盐-Se 处理，范围为 0 至 4 mg kg-1。与成熟时的对照相比，硒处理的各种植物组织中的硒含量显着增加。总可溶性蛋白质和不同的蛋白质组分，即。与对照相比，硒处理的小麦籽粒中的白蛋白、球蛋白、醇溶蛋白和谷蛋白显着降低。相对于蛋白质部分，Se 的含量在谷蛋白部分最高，其次是醇溶蛋白、白蛋白和球蛋白。电泳分析和光密度分析揭示了成熟时不同蛋白质组分中多肽带的存在差异。傅里叶变换红外光谱分析揭示了具有更高亚硒酸盐剂量的结构的更多灵活性和展开。Se 与蛋白质或氨基酸的过度结合可能会取代蛋白质的巯基，从而对正常蛋白质形式产生有害影响。然而，可以利用更高浓度的蛋白质结合硒来强化缺硒食品，为缺硒人群准备富硒饮食和各种保健品。Se 与蛋白质或氨基酸的过度结合可能会取代蛋白质的巯基，从而对正常蛋白质形式产生有害影响。然而，可以利用更高浓度的蛋白质结合硒来强化缺硒食品，为缺硒人群准备富硒饮食和各种保健品。Se 与蛋白质或氨基酸的过度结合可能会取代蛋白质的巯基，从而对正常蛋白质形式产生有害影响。然而，可以利用更高浓度的蛋白质结合硒来强化缺硒食品，为缺硒人群准备富硒饮食和各种保健品。