Iron oxide nanoparticles (NPs) have been a very appealing choice in magnetic-mediated hyperthermia for cancer therapy. The responses of NPs to hyperthermia as a cancer treatment method are complex and variable. Herein, the heating properties of the casein-coated magnetic NPs (MNPs) under an alternating magnetic field were investigated. The casein-coated MNPs were synthesized via one-pot chemical method. The casein-coated MNPs were characterized using transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDAX), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), zeta potential, dynamic light scattering (DLS), and vibrating sample magnetometer (VSM) analysis. TEM images of casein-coated MNPs show that their shapes are spherical and their core sizes are between 20[Formula: see text]nm and 25[Formula: see text]nm. The FTIR and EDAX results confirmed the presence of casein on the surface of MNPs. The VSM shows the superparamagnetic nature of iron oxide and casein-coated iron oxide NPs with the magnetic saturation of 60[Formula: see text]emu/g and 44.86[Formula: see text]emu/g, respectively, at room temperature. Furthermore, hyperthermia tests for casein-coated MNPs with various concentrations and frequencies are performed. Hyperthermia results show that lower concentrations of casein-coated MNPs dispatch higher heating into their surrounding medium, whereas maximum specific absorption rate occurs at the concentration of 1[Formula: see text]mg/mL for the frequency of 150[Formula: see text]kHz. Findings of this study suggest that casein-coated MNPs have great potential as an anticancer agent in hyperthermia cancer therapy.

中文翻译：

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用于癌症治疗的体外热疗的酪蛋白涂层氧化铁纳米颗粒

氧化铁纳米颗粒 (NPs) 一直是用于癌症治疗的磁介导热疗中非常有吸引力的选择。NPs 对作为癌症治疗方法的热疗的反应是复杂且多变的。在此，研究了在交变磁场下涂有酪蛋白的磁性纳米粒子 (MNP) 的加热特性。通过一锅化学法合成酪蛋白包被的MNP。使用透射电子显微镜 (TEM)、扫描电子显微镜 (SEM)、能量色散 X 射线光谱 (EDAX)、傅里叶变换红外光谱 (FTIR)、X 射线衍射 (XRD)、zeta 电位对酪蛋白包被的 MNPs 进行了表征、动态光散射 (DLS) 和振动样品磁力计 (VSM) 分析。酪蛋白包被的 MNPs 的 TEM 图像显示它们的形状是球形的，它们的核心尺寸在 20[公式：见文本]nm 和 25[公式：见文本]nm 之间。FTIR 和 EDAX 结果证实了 MNP 表面存在酪蛋白。VSM 显示了氧化铁和酪蛋白包覆的氧化铁纳米颗粒的超顺磁性，在室温下磁饱和度分别为 60[公式：见文本]emu/g 和 44.86[公式：见文本]emu/g。此外，还对具有不同浓度和频率的酪蛋白涂层 MNP 进行了热疗测试。热疗结果表明，较低浓度的酪蛋白涂层 MNP 将较高的热量散发到其周围介质中，而最大比吸收率发生在 1[公式：见文本]mg/mL 的浓度，频率为 150[公式：见文本]千赫。