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Cellulose Acetate/Magnetic Graphene Nanofiber in Enhanced Human Mesenchymal Stem Cells Osteogenic Differentiation Under Alternative Current Magnetic Field
SPIN ( IF 1.3 ) Pub Date : 2019-04-22 , DOI: 10.1142/s2010324719400113
Shadie Hatamie, Farzaneh Mohamadyar-Toupkanlou, Samaneh Mirzaei, Mohammad Mahdi Ahadian, Simzar Hosseinzadeh, Masoud Soleimani, Wen-Jenn Sheu, Zung Hang Wei, Teng-Fu Hsieh, Wei-Chieh Chang, Chiung-Lang Wang

The three-dimensional (3D) nano scaffold of the cellulose acetate (CA) containing graphene/cobalt nanocomposite (0.1[Formula: see text]wt.%) was fabricated via electrospinning technique, and its impact on bone regeneration was investigated. Through this aim, bone marrow mesenchymal stem cells are cultured on the CA, and graphene/cobalt (rGO/Co)/CA nanocomposite scaffold surfaces and the samples are treated under low frequency alternative magnetic field (75[Formula: see text]Hz). The scaffolds are characterized using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and thermal studies (TG/DSC). The proliferation behavior of stem cells on CA, and rGO/Co/CA nano scaffolds are studied by MTT assay, show their biocompability after 14 days of cell seeding. The 6-diamidino-2-phenylindole (DAPI) staining is used to confirm the morality of stem cell for duration of seven days. The nanocomposite scaffold is enhanced for extremely higher proliferation compared to the bare CA scaffold. The acceleration on osteogenic differentiation on the bone mesenchymal stem cell is enhanced within 48[Formula: see text]h when rGO/Co/CA scaffold is placed under alternative current magnetic field (ACMF). Furthermore, the acceleration of the stem cells differentiation for the rGO/Co/CA scaffold under ACMF corresponds to the induced scaffold surface roughness caused by graphene sheets, the metallic behavior of graphene and the responding of the nanocomposite magnetic parts (i.e., cobalt nanoparticles) while applying 75[Formula: see text]Hz frequency. Using reverse transcription polymerase chain reaction (RT-PCR) analysis, the superior effect of ACMF on scaffold contain magnetic graphene nanocomposite is confirmed to produce bone related genes within 14 days.

中文翻译:

交流磁场下增强人间充质干细胞成骨分化的醋酸纤维素/磁性石墨烯纳米纤维

通过静电纺丝技术制备了含有石墨烯/钴纳米复合材料(0.1[公式:见正文]wt.%)的醋酸纤维素(CA)的三维(3D)纳米支架,并研究了其对骨再生的影响。为此,在CA上培养骨髓间充质干细胞,石墨烯/钴(rGO/Co)/CA纳米复合材料支架表面和样品在低频交变磁场(75[公式:见正文]Hz)下处理. 使用傅里叶变换红外光谱 (FTIR)、X 射线衍射 (XRD) 和热研究 (TG/DSC) 对支架进行表征。通过MTT法研究了干细胞在CA和rGO/Co/CA纳米支架上的增殖行为,显示了它们在细胞接种14天后的生物相容性。6-二脒基-2-苯基吲哚 (DAPI) 染色用于确认干细胞的道德性,持续 7 天。与裸 CA 支架相比,纳米复合支架得到了增强,具有极高的增殖能力。当 rGO/Co/CA 支架置于交流磁场 (ACMF) 下时,骨间充质干细胞上成骨分化的加速在 48 [公式:见正文]h 内得到增强。此外,ACMF下rGO/Co/CA支架的干细胞分化加速对应于石墨烯片引起的诱导支架表面粗糙度、石墨烯的金属行为和纳米复合磁性部件(即钴纳米颗粒)的响应。同时应用 75[公式:见正文]Hz 频率。使用逆转录聚合酶链反应 (RT-PCR) 分析,
更新日期:2019-04-22
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