Abstract

The members of the casein kinase 1 (CK1) subfamily are distinguished by interspecific conservatism and an extensive set of phosphorylation substrates. Recently, appeared strong evidence that CK1 are able to phosphorylate tubulin directly. Thus, CK1 contribute to the tubulin code and functional specialization of microtubules. In this work, we present the results of studying the response of plant tubulin cytoskeleton to the treatment with the CK1-specific inhibitor D4476. It was demonstrated that D4476 shows a strong dose-dependent effect on Arabidopsis thaliana root growth and morphology. The experiments on the plants expressing chimeric gfp-map4 gene construct proved the relation of the observed morphological reactions with the spatial organization of microtubules caused by a selective inhibition of protein kinases CK1.

中文翻译：

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研究蛋白激酶CK1在拟南芥根细胞皮层微管组织中的作用。

摘要

酪蛋白激酶1（CK1）亚家族的成员以种间保守性和广泛的磷酸化底物为特征。最近，出现了有力的证据表明CK1能够直接磷酸化微管蛋白。因此，CK1有助于微管蛋白代码和微管的功能专业化。在这项工作中，我们介绍了研究植物微管蛋白细胞骨架对CK1特异性抑制剂D4476的反应的结果。结果表明，D4476对拟南芥根的生长和形态表现出强烈的剂量依赖性。表达嵌合gfp-map4的植物的实验 基因构建证明了观察到的形态反应与蛋白激酶CK1的选择性抑制引起的微管空间组织的关系。