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Comparative Analysis of Paddlefish ( Polyodon spathula ) Populations’ Genetic Structure with Regard to Microsatellite DNA Markers
Cytology and Genetics ( IF 0.5 ) Pub Date : 2020-02-10 , DOI: 10.3103/s0095452720010107 Kh. M. Kurta , O. O. Malysheva , V. G. Spyrydonov
中文翻译:
基于微卫星DNA标记的鳞茎鱼类(Polyodon spathula)种群遗传结构的比较分析
更新日期:2020-02-10
Cytology and Genetics ( IF 0.5 ) Pub Date : 2020-02-10 , DOI: 10.3103/s0095452720010107 Kh. M. Kurta , O. O. Malysheva , V. G. Spyrydonov
Abstract
Comparative characteristics in genetic structure of cultured Ukrainian and Polish populations and the natural paddlefish populations from the United States is reported for three microsatellite DNA markers: Psp21, Psp26, and Psp28. The average Na value was 6.1 and 5.5 for the Ukrainian and Polish populations, respectively. The Na value for the natural populations was almost two times higher at 11.1 alleles on average. The mean values of observed (Но) heterozygosity were shown to exceed those of expected heterozygosity (Не) for both Ukrainian (0.709 > 0.616) and Polish (0.809 > 0.699) populations. The mean Но and Не values for the natural populations were in the range of genetic equilibrium according to the Hardy–Weinberg equation at 0.817 and 0.813, respectively. According to the data presented, changes in the total number of allelic variants in the cultured populations as compared to natural populations were demonstrated. The heterozygosity level values obtained and negative values of the fixation index Fis for the cultured populations point at the absence of inbreeding at the present stage of paddlefish cultivation, and this is indicative of a sufficient number of breeders with a heterozygous genotype for reproduction under aquaculture conditions.中文翻译:
基于微卫星DNA标记的鳞茎鱼类(Polyodon spathula)种群遗传结构的比较分析