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Comparative Analysis of Paddlefish ( Polyodon spathula ) Populations’ Genetic Structure with Regard to Microsatellite DNA Markers
Cytology and Genetics ( IF 0.5 ) Pub Date : 2020-02-10 , DOI: 10.3103/s0095452720010107
Kh. M. Kurta , O. O. Malysheva , V. G. Spyrydonov

Abstract

Comparative characteristics in genetic structure of cultured Ukrainian and Polish populations and the natural paddlefish populations from the United States is reported for three microsatellite DNA markers: Psp21, Psp26, and Psp28. The average Na value was 6.1 and 5.5 for the Ukrainian and Polish populations, respectively. The Na value for the natural populations was almost two times higher at 11.1 alleles on average. The mean values of observed (Но) heterozygosity were shown to exceed those of expected heterozygosity (Не) for both Ukrainian (0.709 > 0.616) and Polish (0.809 > 0.699) populations. The mean Но and Не values for the natural populations were in the range of genetic equilibrium according to the Hardy–Weinberg equation at 0.817 and 0.813, respectively. According to the data presented, changes in the total number of allelic variants in the cultured populations as compared to natural populations were demonstrated. The heterozygosity level values obtained and negative values of the fixation index Fis for the cultured populations point at the absence of inbreeding at the present stage of paddlefish cultivation, and this is indicative of a sufficient number of breeders with a heterozygous genotype for reproduction under aquaculture conditions.


中文翻译:

基于微卫星DNA标记的鳞茎鱼类(Polyodon spathula)种群遗传结构的比较分析

摘要

据报道,三种微卫星DNA标记物:Psp21,Psp26和Psp28在来自乌克兰和波兰的人工养殖种群和来自美国的天然河豚种群的遗传结构中具有比较特征。乌克兰和波兰人口的平均Na值分别为6.1和5.5。自然种群的Na值平均为11.1个等位基因,几乎高出两倍。对于乌克兰(0.709> 0.616)和波兰(0.809> 0.699)人群,观察到的(Но)杂合度平均值均超过了预期的杂合度(Не)。平均值НоНе根据Hardy-Weinberg方程,自然种群的数值分别处于遗传平衡范围内,分别为0.817和0.813。根据提供的数据,证明了与自然种群相比,培养种群的等位基因变体总数发生了变化。所获得的杂合度水平值和养殖种群的固定指数Fis的负值表明在桨鱼养殖的现阶段没有近亲繁殖,这表明有足够数量的杂合基因型育种者可以在水产养殖条件下繁殖。
更新日期:2020-02-10
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