In southern Australia, oriental mustard (Sisymbrium orientale) has been controlled successfully by triazine herbicides for several decades. The screening of 40 populations that were collected from the southern grain belt of Australia during 2010 and 2013 for resistance to six different herbicides (glyphosate, diflufenican, imazamox, chlorsulfuron, atrazine and 2,4-dichlorophenoxyacetic acid) identified two oriental mustard populations as highly resistant to atrazine. Compared to the known oriental mustard-susceptible populations (S1 and S2), these two resistant populations (P17 and P18) from near Horsham, Victoria, Australia, were 311- and 315-fold resistant to atrazine, as determined by a comparison of the LD50 values. However, there was no resistance to diuron detected in these populations. Sequencing of the chloroplast psbA gene identified a missense mutation of serine 264 to glycine in both herbicide-resistant oriental mustard populations, which is known to confer high-level atrazine resistance in other species.

中文翻译：

---

在来自澳大利亚的东方芥 (Sisymbrium orientale L.) 中鉴定出赋予三嗪类除草剂抗性的靶位点突变

在澳大利亚南部，几十年来，三嗪类除草剂已成功控制了东方芥菜 (Sisymbrium orientale)。对 2010 年和 2013 年期间从澳大利亚南部粮食带收集的 40 个种群进行的对六种不同除草剂（草甘膦、双氟苯甲胺、咪草啶酸、氯磺隆、莠去津和 2,4-二氯苯氧乙酸）的抗性筛选发现，两个东方芥菜种群具有高度的抗性。耐阿特拉津。与已知的东方芥末易感种群（S1 和 S2）相比，来自澳大利亚维多利亚州霍舍姆附近的这两个抗性种群（P17 和 P18）对莠去津的抗性是 311 倍和 315 倍，这是通过比较LD50 值。然而，在这些人群中没有检测到对敌草隆的抗性。