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Seed storage protein phylogenetics of Indian wheat genotypes belong to Triticum aestivum, T. dicoccum and T. durum
Indian Journal of Biochemistry and Biophysics ( IF 1.476 ) Pub Date : 2020-09-21
Monika Sihmar, Jitendra Kumar Sharma, Anita Rani Santal, N. P. Singh

Genetic diversity analysis of eighty six Indian wheat genotypes was performed using sodium dodecyl sulphate polyacrylamide gel (SDS-PAGE) electrophoresis based on their total seed storage protein. A dendrogram was constructed using the genetic similarity coefficient matrix based on SDS-PAGE, which categorized these genotypes according to their electrophoretic patterns into eight different clusters. These eighty six genotypes studied here belong to three different species of wheat. Out of these eighty six genotypes, two genotypes belong to T. dicoccum; eleven belong to T. durum and the remaining seventy three belongs to T. aestivum. In the dendrogram, all the eleven genotypes of the species T. durum (A-9-30-1, HD-4672, RAJ-1555, PDW-233, PBW-34, HI-8381, PDW-291, WH-896, PDW-215, HI-8498 and MACS-2846) were grouped in one cluster. The pedigree analysis of genotypes showed similar parentage with the electrophoretic profiling of the different wheat genotypes. From this study, it has been concluded that the total seed storage protein profile of wheat can be used as a marker for genetic diversity.

中文翻译:

印度小麦基因型种子贮藏蛋白的系统发育研究属于普通小麦,T。dicoccum和T. durum

基于总种子贮藏蛋白,使用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)进行了86种印度小麦基因型的遗传多样性分析。使用基于SDS-PAGE的遗传相似性系数矩阵构建树状图,该树状图根据这些基因型的电泳模式将其分为八个不同的簇。此处研究的这八十六个基因型属于三种不同的小麦。在这86种基因型中,有2种属于T. dicoccum。十一个属于杜伦木T. durum),其余的七个属于小麦T. aestivum)。在树状图中,杜氏杜鹃种的所有十一个基因型(A-9-30-1,HD-4672,RAJ-1555,PDW-233,PBW-34,HI-8381,PDW-291,WH-896,PDW-215,HI-8498和MACS-2846)是归为一类。基因型的谱系分析显示,不同小麦基因型的电泳谱图具有相似的亲缘关系。根据这项研究,可以得出结论,小麦的总种子贮藏蛋白特征可以用作遗传多样性的标记。
更新日期:2020-09-21
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