We estimated the effect of residual lignin and pulp swelling on the rate of enzymatic saccharification to increase production of ethanol from acid sulfite pulp (SP) by means of enzymatic treatment. The resolution ratio of hardwood (Acacia mearnsii) SP after the enzymatic treatment was lower compared to softwood (Larix leptolepis) SP even though lignin content of hardwood SP was lower. The pyrolysis–gas chromatography/mass spectrometry (Py-GC/MS) analysis revealed that the residual lignin in hardwood SP could more easily adsorb enzyme compared to softwood SP, and the residual lignin in hardwood SP should interfere with the binding of the enzyme to cellulose. The beating treatment of pulp increased the swelling of pulp. The enzymatic saccharification rate was increased by the beating treatment. On the other hand, the delignification treatment was more effective than the beating treatment at enhancing enzymatic saccharification of both hardwood and softwood SPs. We found that the delignification process should be considered a high-priority technique for enhancing enzymatic saccharification of SP.

中文翻译：

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残留木质素和纤维素膨胀对软木和硬木酸性亚硫酸盐纸浆酶促糖化速率的影响

我们估计了残留的木质素和果肉膨胀对酶促糖化速率的影响，以通过酶处理增加酸性亚硫酸盐果肉（SP）的乙醇产量。即使硬木SP的木质素含量较低，酶处理后的硬木（Acacia mearnsii）SP的拆分率也比软木（Larix leptolepis）SP低。热解-气相色谱/质谱（Py-GC / MS）分析显示，硬木SP中残留的木质素比软木SP更容易吸附酶，并且硬木SP中的残留木质素应干扰酶与木质素的结合。纤维素。纸浆的打浆处理增加了纸浆的溶胀。通过打浆处理提高了酶促糖化率。另一方面，在增强硬木和软木SP的酶促糖化作用方面，脱木素处理比打浆处理更有效。我们发现，脱木素过程应被认为是增强SP的酶糖化的高优先技术。