Lilac (Syringa oblata) is an important woody plant with high ornamental value. However, very limited genetic marker resources are currently available, and little is known about the genetic architecture of important ornamental traits for S. oblata, which is hindering its genetic studies. Therefore, it is of great significance to develop effective molecular markers and understand the genetic architecture of complex floral traits for the genetic research of S. oblata. In this study, a total of 10,988 SSRs were obtained from 9864 unigene sequences with an average of one SSR per 8.13 kb, of which di-nucleotide repeats were the dominant type (32.86%, 3611). A set of 2042 primer pairs were validated, out of which 932 (45.7%) exhibited successful amplifications, and 248 (12.1%) were polymorphic in eight S. oblata individuals. In addition, 30 polymorphic EST-SSR markers were further used to assess the genetic diversity and the population structure of 192 cultivated S. oblata individuals. Two hundred thirty-four alleles were detected, and the PIC values ranged from 0.23 to 0.88 with an average of 0.51, indicating a high level of genetic diversity within this cultivated population. The analysis of population structure showed two major subgroups in the association population. Finally, 20 significant associations were identified involving 17 markers with nine floral traits using the mixed linear model. Moreover, marker SO104, SO695 and SO790 had significant relationship with more than one trait. The results showed newly developed markers were valuable resource and provided powerful tools for genetic breeding of lilac. Beyond that, our study could serve an efficient foundation for further facilitate genetic improvement of floral traits for lilac.

中文翻译：

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紫丁香EST-SSR标记的开发和与花性状的关联图谱。

丁香（Syringa oblata）是一种重要的木本植物，具有很高的观赏价值。然而，目前可用的遗传标记资源非常有限，关于扁圆形链球菌重要观赏性状的遗传结构知之甚少，这阻碍了其遗传研究。因此，开发有效的分子标记和了解复杂花卉性状的遗传结构对扁叶酵母的遗传学研究具有重要意义。在这项研究中，从9864个单基因序列中总共获得了10988个SSR，平均每8.13 kb有一个SSR，其中二核苷酸重复序列占主导地位（32.86％，3611）。验证了一组2042个引物对，其中有932个（45.7％）表现出成功的扩增，并且在8个扁圆形链球菌个体中有248个（12.1％）多态性。此外，30个多态性EST-SSR标记进一步用于评估192个培养的扁豆链球菌个体的遗传多样性和种群结构。检测到234个等位基因，PIC值在0.23至0.88之间，平均值为0.51，表明该耕种群体中遗传多样性高。人口结构分析显示，协会人口中有两个主要的亚群。最后，使用混合线性模型确定了20个重要的关联，涉及17个具有9个花性状的标记。而且，标记SO104，SO695和SO790与一种以上的性状具有显着的关系。结果表明，新开发的标记是有价值的资源，为丁香的遗传育种提供了有力的工具。除此之外，