Sweetpotato (Ipomoea batatas L.) is one of the seven major food crops grown worldwide. Cold stress often can cause protein expression pattern and substance contents variations for tuberous roots of sweetpotato during low-temperature storage. Recently, we developed proteometabolic profiles of the fresh sweetpotatoes (cv. Xinxiang) in an attempt to discern the cold stress-responsive mechanism of tuberous root crops during post-harvest storage. For roots stored under 4 °C condition, the CI index, REC and MDA content in roots were significantly higher than them at control temperature (13 °C). The activities of SOD, CAT, APX, O2.- producing rate, proline and especially soluble sugar contents were also significantly increased. Most of the differentially expressed proteins (DEPs) were implicated in pathways related to metabolic pathway, especially phenylpropanoids and followed by starch and sucrose metabolism. L-ascorbate peroxidase 3 and catalase were down-regulated during low temperature storage. α-amylase, sucrose synthase and fructokinase were significantly up-regulated in starch and sucrose metabolism, while β-glucosidase, glucose-1-phosphate adenylyl-transferase and starch synthase were opposite. Furthermore, metabolome profiling revealed that glucosinolate biosynthesis, tropane, piperidine and pyridine alkaloid biosynthesis as well as protein digestion and absorption played a leading role in metabolic pathways of roots. Leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis. Our proteomic and metabolic profile analysis of sweetpotatoes stored at low temperature reveal that the antioxidant enzymes activities, proline and especially soluble sugar content were significantly increased. Most of the DEPs were implicated in phenylpropanoids and followed by starch and sucrose metabolism. The discrepancy between proteomic (L-ascorbate peroxidase 3 and catalase) and biochemical (CAT/APX activity) data may be explained by higher H2O2 levels and increased ascorbate redox states, which enhanced the CAT/APX activity indirectly. Glucosinolate biosynthesis played a leading role in metabolic pathways. Leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis.

中文翻译：

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番薯叶低温贮藏反应的蛋白质组和代谢谱分析。块茎根。

甘薯（Ipomoea batatas L.）是世界上种植的七种主要粮食作物之一。低温胁迫通常会导致低温贮藏期间甘薯块根的蛋白质表达模式和物质含量变化。最近，我们开发了新鲜甘薯（cv。Xinxiang）的蛋白质代谢谱，以期在收获后的贮藏过程中识别块根作物的冷胁迫响应机制。对于在4°C条件下存储的根，其CI指数，REC和MDA含量均显着高于对照温度（13°C）。SOD，CAT，APX，O2-的活性，产酸率，脯氨酸，尤其是可溶性糖含量也显着增加。大多数差异表达蛋白（DEP）都与代谢途径有关，尤其是苯丙烷，然后发生淀粉和蔗糖代谢。L-抗坏血酸过氧化物酶3和过氧化氢酶在低温储存过程中被下调。淀粉和蔗糖代谢中α-淀粉酶，蔗糖合酶和果糖激酶显着上调，而β-葡萄糖苷酶，葡萄糖-1-磷酸腺苷基转移酶和淀粉合酶则相反。此外，代谢组学分析表明，芥子油苷的生物合成，托烷，哌啶和吡啶生物碱的生物合成以及蛋白质的消化吸收在根的代谢途径中起着主导作用。芥子油苷的生物合成中，亮氨酸，色氨酸，酪氨酸，异亮氨酸和缬氨酸均显着上调。我们对低温贮藏的甘薯的蛋白质组学和代谢谱分析表明，抗氧化酶的活性 脯氨酸，尤其是可溶性糖含量显着增加。大多数DEP与苯丙烷有关，随后与淀粉和蔗糖代谢有关。蛋白质组学数据（L-抗坏血酸过氧化物酶3和过氧化氢酶）与生化数据（CAT / APX活性）之间的差异可以通过较高的H2O2水平和增加的抗坏血酸氧化还原态来解释，这间接提高了CAT / APX活性。芥子油苷的生物合成在代谢途径中起主要作用。芥子油苷的生物合成中，亮氨酸，色氨酸，酪氨酸，异亮氨酸和缬氨酸均显着上调。蛋白质组学数据（L-抗坏血酸过氧化物酶3和过氧化氢酶）与生化数据（CAT / APX活性）之间的差异可以通过较高的H2O2水平和增加的抗坏血酸氧化还原态来解释，这间接提高了CAT / APX活性。芥子油苷的生物合成在代谢途径中起主要作用。芥子油苷的生物合成中，亮氨酸，色氨酸，酪氨酸，异亮氨酸和缬氨酸均显着上调。蛋白质组学数据（L-抗坏血酸过氧化物酶3和过氧化氢酶）与生化数据（CAT / APX活性）之间的差异可以通过较高的H2O2水平和增加的抗坏血酸氧化还原态来解释，这间接提高了CAT / APX活性。芥子油苷的生物合成在代谢途径中起主要作用。芥子油苷的生物合成中，亮氨酸，色氨酸，酪氨酸，异亮氨酸和缬氨酸均显着上调。