This study determined the influence of photoperiod on the expression of toll-like receptor 2 and 4 (TLR2 and TLR4), interleukin 1□ (IL1B), IL-1 receptor type I (IL1R1) and II (IL1R2), interleukin 6 (IL6), the IL-6 receptor (IL6R) and signal transducer (IL6ST), tumor necrosis factor α (TNF), and TNF□ receptor type I (TNFRSF1A) and II (TNFRSF1B) in the choroid plexus (ChP) of ewes with lipopolysaccharide (LPS)-induced acute inflammation. Under short-days (SD, n = 12, anestrous) and long-days (LD, n = 12, synchronized follicular phase), ewes were treated with saline or LPS. Compared to LD conditions, the ewes under SD were characterized by a greater (P<0.05) area under the curve (AUC) of cortisol in the LPS-treated group and by a lower (P<0.05) AUC of prolactin in the saline-treated group. Under both photoperiods, LPS increased (P<0.05) the expression of all examined genes except for TNFRSF1B (only under SD), TNF and TNFRSF1A (no stimulation), and IL6R (decreased (P<0.05) under SD). The LPS-induced increases in TLR2, TLR4, IL1B and its receptors, IL6 and TNFRSF1B were higher (P<0.05) under SD than LD. TLR4 was positively correlated with IL1B and IL6 in both saline- (r² = 0.64, P<0.01 and r² = 0.52, P<0.01) and LPS-treated (r² = 0.81, P<0.0001 and r² = 0.51, P<0.001) ewes. IL1B (r² = 0.56, P<0.01 and r² = 0.77, P<0.0001) and IL6 (r² = 0.77, P<0.005 and r² = 0.35, P<0.05) were positively correlated with TLR2 in saline- and LPS-treated ewes, respectively. This indicates that in ewes, the ChP response to acute systemic inflammation is dependent upon the photoperiod with stronger effects being observed under SD. Our results also suggest that gonadal hormones altering TLR4 signaling events are involved in the photoperiodic modulation of the ChP response to LPS. Further experiments are required to explain the mechanism involved in this phenomenon.

中文翻译：

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光周期改变了对脂多糖诱导的母羊急性炎症的脉络丛反应

这项研究确定了光周期对收费型受体2和4（TLR2和TLR4），白介素1□（IL1B），IL-1受体I（IL1R1）和II（IL1R2），白介素6（IL6）表达的影响。）中，IL-6受体（IL6R）和信号转导（IL6ST），肿瘤坏死因子α（TNF）和TNF受体□I型（TNFRSF1A）和II（TNFRSF1B）与脂多糖（LPS）诱导的急性炎症的母羊脉络丛（ChP）中。在短日（SD，n = 12，动情期）和长日（LD，n = 12，同步卵泡期）下，母羊用生理盐水或LPS处理。与LD条件相比，SD下的母羊在LPS治疗组中的皮质醇曲线下面积（AUC）较大（P <0.05），在盐水中的催乳素的AUC较低（P <0.05）。治疗组。在两种光周期下，除了TNFRSF1B（仅在SD下），TNF和TNFRSF1A（无刺激）和IL6R（在SD下降低（P <0.05））之外，LPS均提高了所有受检基因的表达（P <0.05）。LPS诱导的TLR2和TLR4升高SD时，IL1B及其受体IL6和TNFRSF1B高于LD（P <0.05）。在生理盐水中-（r ² = 0.64，P <0.01和r ² = 0.52，P <0.01）和LPS处理（TL ² = 0.81，P <0.0001和r ² = 0.51，TLR4与IL1B和IL6正相关， P <0.001）母羊。IL1B（r ² = 0.56，P <0.01和r ² = 0.77，P <0.0001）和IL6（r ² = 0.77，P <0.005和r ² = 0.35，P <0.05）与TLR2正相关分别在生理盐水和LPS处理的母羊中。这表明在母羊中，ChP对急性全身性炎症的反应取决于光周期，在SD下观察到更强的作用。我们的结果还表明，改变TLR4信号事件的性腺激素与ChP对LPS反应的光周期调节有关。需要进一步的实验来解释这种现象的机制。