The aim of this study was to examine the effect of thymosin beta 4 (Tβ4) during in vitro maturation (IVM) of oocytes and subsequent embryonic development after in vitro fertilization as well as to assess the quality of obtained blastocysts. Cumulus-oocyte complexes (COCs) were matured in vitro in 4 different media: 1. control medium; 2. control media supplemented with 50 ng/mL Tβ4; 3. control media supplemented with 0.5 mg/mL Tβ4; and 4. control media supplemented with 1 mg/mL Tβ4. The quality of the developed blastocysts was analysed by the TUNEL method. The number of cleaved eggs was significantly higher (P<0.05) when gametes were matured in the presence of 50 ng/mL Tβ4 than it was using the other types of media. Additionally, the largest number of blastocysts was observed when 0.5 mg Tβ4 was added to the medium (P<0.05). No significant difference was noted in the mean number of apoptotic nuclei per blastocyst or in the mean number of nuclei per blastocyst in any of the analysed groups. In conclusion, Tβ4 supplementation (50 ng/mL) in maturation medium increased the number of cleaved oocytes, and the number of blastocysts obtained increased when 0.5 mg/mL Tβ4 was used. This positive effect was not observed when a higher concentration of Tβ4 (1 mg/mL) was used.

中文翻译：

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胸腺素β4在体外成熟的牛卵母细胞的发育能力

本研究的目的是在检查胸腺素β4（Tβ4）的影响的体外后卵母细胞和早期胚胎发育的成熟（IVM）体外授精以及评估获得胚泡的质量。卵丘卵母细胞复合物（COCs）在体外成熟在4种不同的介质中：1.控制介质；2.对照培养基中添加50 ng / mLTβ4；3.补充0.5 mg / mLTβ4的对照培养基；4.补充有1mg / mLTβ4的对照培养基。通过TUNEL方法分析发育中的胚泡的质量。当配子在50 ng / mLTβ4存在下成熟时，裂解卵的数量显着高于使用其他类型的培养基（P <0.05）。另外，当向培养基中添加0.5 mgTβ4时，观察到的胚泡数量最多（P <0.05）。在任何分析组中，每个胚泡的凋亡核平均数或每个胚泡的平均核数均无显着差异。总之，在成熟培养基中补充Tβ4（50 ng / mL）可以增加卵母细胞裂解的数量，当使用0.5 mg / mLTβ4时，获得的胚泡数目增加。当使用更高浓度的Tβ4（1 mg / mL）时，未观察到这种积极作用。